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Method for artificial infestation of Odontothrips loti on alfalfa

A technology for hornflower and thrips nymphs, which is applied in the fields of botanical equipment and methods, horticulture, and applications, can solve the problems of large escape and mortality, difficult operation, time-consuming and labor-intensive, etc., and achieves that the method is easy to operate and highly controllable. sex, low mortality

Inactive Publication Date: 2012-07-11
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Thrips cerevisiae lays eggs in the leaves of alfalfa, and its newly hatched nymphs are extremely small and fragile. plants), this method is relatively feasible when the indoor alfalfa requires a low population of C. Thrips are tiny and prone to errors
Using this method to pick up worms in the field, no matter whether it is an adult or a nymph, it cannot be operated
Although it is possible to quantify the population, this method is very time-consuming and labor-intensive, and the mortality rate of Thrips cerevisiae is also high, so it is especially difficult to operate in the field
In addition, if the beaker quantitative inoculation method of aphids is used, it will cause greater escape and mortality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Inoculation methods for nymphs of Thrips cerevisiae.

[0022] (1) Precise number of nymphs of Thrips ceriferata were acquired

[0023] Place several newly expanded leaves of alfalfa in a single layer radially in a glass culture dish of φ90 mm, keep the petioles moist with cotton balls, insert 20 female adults of Thrips cerevisiae with a brush, and seal them with a single-layer fresh-keeping bag for cultivation Dish to prevent the female adults of Thrips cerevisiae from escaping, and then use a No. 1 insect needle to pierce several small holes on it to maintain ventilation and prevent water accumulation. Place it in an artificial climate box at 24.5±1°C, photoperiod L:D=14:10h, keep the relative air humidity at 60%, let the female adults of Thrips cerifera lay eggs on the newly expanded leaves of alfalfa, and after 1 day Removal of adult female thrips cerevisiae. Under the 4 times eyepiece of the microscope, quickly count the number of eggs of the female adults of Thri...

Embodiment 2

[0027] Inoculation methods for nymphs of Thrips cerevisiae.

[0028] (1) Approximate number of nymph populations of Thrips cerevisiae were acquired

[0029] Place 15 newly expanded leaves of alfalfa radially in a φ150mm glass petri dish, keep the petioles moist with cotton balls, insert 15 female adults of Thrips cerevisiae with a brush, and seal the petri dish with a single-layer fresh-keeping bag to prevent the horns The female adults of Thrips dentata escaped, and then used a No. 1 insect needle to pierce several small holes on it to maintain ventilation and prevent stagnant water. Place it in an artificial climate box at 24.5±1°C, photoperiod L:D=14:10h, keep the relative air humidity at 60%, let the female adults of Thrips cerifera lay eggs on the newly expanded leaves of alfalfa, and after 1 day Remove the female adults of Thrips cerevisiae, and culture them under the conditions of 24.5±1°C, photoperiod L: D=14: 10h, and relative air humidity of 60%. After 4 to 5 days, ...

Embodiment 3

[0033] Inoculation methods for nymphs of Thrips cerevisiae.

[0034] (1) Approximate number of nymph populations of Thrips cerevisiae were acquired

[0035] Place 30 newly expanded leaves of alfalfa radially in a glass petri dish of φ150 mm, keep the petioles moist with cotton balls, insert 20 female adults of Thrips cerevisiae with a brush, and seal the petri dish with a single-layer fresh-keeping bag to prevent the horns The female adults of Thrips dentata escaped, and then used a No. 1 insect needle to pierce several small holes on it to maintain ventilation and prevent stagnant water. Place it in an artificial climate box at 24.5±1°C, photoperiod L: D=14: 10h, keep the relative air humidity at 60%, let the female adults of Thrips ceriferata lay eggs on the leaves of alfalfa, and remove them after 1 day The female adults of Thrips cerevisiae are cultivated under the conditions of 24.5±1°C, photoperiod L: D=14: 10h, and relative air humidity of 60%. After 4 to 5 days, 150-1...

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Abstract

The invention provides a method for artificial infestation of Odontothrips loti on alfalfa, wherein the method for artificial infestation of Odontothrips loti nymphs comprises placing alfalfa newly expanded leaves in culture dishes, inoculating Odontothrips loti female imagoes, sealing the culture dishes with perforated fresh-keeping bags, culturing under certain conditions to obtain Odontothrips loti nymphs, and placing nymphs together with the newly expanded leaves to alfalfa plant top parts according to the required insect number of infestation for an individual alfalfa plant. The method for artificial infestation of Odontothrips loti imago comprises putting alfalfa young shoots in a wide-mouthed bottle, inoculating Odontothrips loti imagoes, directly applying on alfalfa plants to be infested, and keeping the wide-mouthed bottle near the plants for a moment. The method of the invention has the following effects: the cost of materials is low, the method is simple, the obtained Odontothrips loti imagoes are uniform and highly viable, the escape rate and the mortality rate of imagoes and nymphs are very low, and the controllability is high. The method is suitable for artificial infestation in a wide range.

Description

technical field [0001] The invention relates to a method for inoculating thrips cerifera on alfalfa. Background technique [0002] Thrips oxhorn ( Odontothrips loti ) is one of the main pests in China's alfalfa producing areas, especially posing a serious threat to the second and third stubbles of northern alfalfa. How to effectively control Thrips cereus has become one of the important issues in the development of my country's alfalfa industry. The body of Thrips ceriferae is small, the body length of newly hatched nymphs is less than 0.4mm, and the width is about 0.1mm, and the body length of adults is about 1-2mm, and the body width is about 0.4mm. The overlap is serious, and the duration of each worm state is very short, and it only takes about 25 days to reproduce one generation. The optimal average temperature for the development and reproduction of Thrips ceriferata is 20~25℃, and the relative humidity is 60~70%. The prevalence rate of alfalfa species in my countr...

Claims

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Application Information

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IPC IPC(8): A01K67/033A01G1/00
Inventor 胡桂馨师尚礼景康康寇江涛王小珊
Owner GANSU AGRI UNIV
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