Preparation method of 10-vanilloyl catalpol

Abstract

The invention discloses a preparation method of a 10-vanilloyl catalpol. The preparation method of the 10-vanilloyl catalpol comprises the following steps of 1, preparing figwortflower picrorhiza rhizome powder and putting it aside for next use, 2, preparing gauze bags and putting then aside for next use, 3, putting the figwortflower picrorhiza rhizome powder into the gauze bags to obtain drug bags with the figwortflower picrorhiza rhizome powder, 4, putting the drug bags with the figwortflower picrorhiza rhizome powder into an extraction container, carrying out extraction, and merging filtrate to obtain an ethanol extracted liquid of figwortflower picrorhiza rhizome, 5, standing the ethanol extracted liquid of figwortflower picrorhiza rhizome, 6, feeding the ethanol extracted liquid of figwortflower picrorhiza rhizome into a plate-frame-type filter press by a pump and carrying out filter pressing to obtain ethanol filtrate, 7, putting the ethanol filtrate into a distiller and carrying out distillation to obtain ethanol extract, 8, adding the ethanol extract into chromatography silica gel to obtain mixed silica gel powder for next use, 9, adding eluants into chromatography silica gel, 10, adding the mixed silica gel powder obtained by the step 8 into a chromatography column and carrying out immersion, 11, carrying out chromatography elution, 12 preparing eluants of ethyl acetate and ethanol and carrying out treatment to obtain a discolored liquid, and 13, carrying out distillation of the discolored liquid to recover a solvent and to obtain ethanol extract, mixing the ethanol extract and silica gel, and cooling to obtain the precipitated 10-vanilloyl catalpol. The preparation method provided by the invention realizes extraction of 10-vanilloyl catalpol of figwortflower picrorhiza rhizome and improves an extraction rate.

Description

technical field [0001] The invention relates to traditional Chinese medicine, and relates to a preparation method for extracting 10-coumaroyl catalpol from raw materials of traditional Chinese medicine. Background technique [0002] 10-coumaryl catalpol exists in the traditional Chinese medicine raw material Huhuanglian, mainly in the bast fiber cells of the rhizome of Huhuanglian. Since the hardness, toughness and compactness of the bast fiber cells of the rhizome of Huhuanglian are much higher than that of the bast fiber cells of the rhizomes of traditional Chinese medicine plants, it is relatively difficult to extract 10-coumaryl catalpol from Huhuanglian. The best published method for extracting 10-coumaryl catalpol in Coptis chinensis without harmful solvent residues can only extract 30% of the total amount of 10-coumaroyl catalpol in the Chinese beetroot. The rate is low, causing a large amount of waste of the active ingredients of Coptis chinensis and the medicinal ...

AI Technical Summary

Problems solved by technology

The best published method for extracting 10-coumaryl catalpol in Coptis chinensis without harmful solvent residues can only extract 30% of the total amount of 10-coumaroyl catalpol in the Chinese beetroot. The rate is lower, causing a large amount of waste of Coptis chinensis active ingredients and Coptis chinensis medicinal materials, resulting in an increase in the cost of medicines prepared with 10-coumaroyl catalpol