Method for acquiring two-dimensional distribution of sediment dissolved reactive phosphorus (DRP)
A technology of two-dimensional distribution and reactive phosphorus, which is applied in the fields of environmental science and earth science, can solve problems such as lack of measurement methods, failure to meet the requirements of DGT high-resolution analysis, and no high-resolution acquisition method yet, to achieve uniform and dense distribution, high resolution effect
Active Publication Date: 2012-06-20
NANJING INST OF GEOGRAPHY & LIMNOLOGY
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Problems solved by technology
Currently commonly used fixatives (such as Chelex 100) have a particle size greater than 0.05mm, which cannot meet the high-resolution analysis requirements of DGT
[0008] DGT technology has been used to obtain the vertical one-dimensional and conventional resolution (>1mm) distribution information of sediment DRP. Due to the limitation of resolution, it is difficult to obtain the two-dimensional distribution information of DRP within the acceptable error range; to obtain the two-dimensional distribution of DRP The information also involves the analysis of phosphorus content in a large number of trace samples, and the existing determination method is not yet available, so there is no report on the high-resolution acquisition method of the two-dimensional distribution information of sediment DRP by this technology
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[0043] According to the method of the present invention, the two-dimensional distribution data of dissolved reactive phosphorus (DRP) in the sediments of Taihu Lake is obtained.
[0044] Two sediment samples were collected from Dapukou of Taihu Lake (31°18′42.7″N, 119°56′52.2″E). After they were transported back to the laboratory, the sediments were stratified by 2cm each layer, and each The layer sediments are thoroughly mixed, and after the benthic animals are removed through a 100-mesh sieve, the sediments are re-packed in the original order, the surface is covered with lake water, and they are cultured indoors. After half a month, put 20 water silkworms into one of the sample columns. After culturing for one month, insert the two DGT devices into the two sample columns respectively. After 6 days, take them out for analysis, and obtain the interface to the area 3cm below the interface. Two-dimensional distribution information of DRP content, and make a two-dimensional distribu...
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Abstract
The invention discloses a method for acquiring two-dimensional distribution of sediment dissolved reactive phosphorus (DRP), which comprises: vertically placing a diffusion gradient in thin-films (DGT) device containing a modified phosphorus immobilizing film in a sediment to extract the DRP in the section of the sediment by using modified ZrO2 polyacrylamide gel thin film, in which the particle size of zirconium dioxide is less than or equal to 5mu m, as a phosphorus immobilizing film based on a DGT principle, and keeping 3 to 5 centimeters of overlying water; marking a sediment-water interface, taking out the immobilizing film, and performing two-dimensional and submillimeter slicing on the part below the interface; and extracting the DRP in the slices by using NaOH, measuring the DRP content in the extract by using microcolorimetry, converting the DRP content into the DRP solid content in the slices, calculating the DRP concentration at the corresponding position in the section of the sediment based on Fick's first law of diffusion, and drawing a two-dimensional distribution map.
Description
Technical field [0001] The invention belongs to the fields of environmental science and earth science, and relates to a method for obtaining the distribution of pollutant content in river, lake and marine sediments, and in particular to a method for obtaining two-dimensional distribution of dissolved reactive phosphorus (DRP) in sediments. Background technique [0002] In aquatic ecosystems, phosphorus is the most important source of primary productivity and food chain, and it is also a limiting factor for eutrophication of water bodies. When the input of external phosphorus is contained, phosphorus pollution in sediments is the main cause of eutrophication of water bodies. Dissolved reactive phosphorus (DRP) is the phosphorus measured by the molybdenum blue colorimetric method. Most of it is phosphate, and it also includes a small amount of organic phosphorus and polyphosphorus compounds that can be hydrolyzed under acidic conditions. DRP is the most active phosphorus in sedime...
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IPC IPC(8): G01N21/31
Inventor 丁士明贾飞许笛孙琴
Owner NANJING INST OF GEOGRAPHY & LIMNOLOGY
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