Genetic transformation method for agrobacterium tumefacien-mediated fusarium oxysporum sesame specialized strain

Abstract

The invention relates to a genetic transformation method for an agrobacterium tumefacien-mediated fusarium oxysporum sesame specialized strain. The genetic transformation method comprises the following steps of: (1) preparing conidiospore suspension of the fusarium oxysporum sesame specialized strain; and (2) performing the transforming-deoxyribonucleic acid (T-DNA) conversion of the fusarium oxysporum sesame specialized strain. According to the genetic transformation method, the fusarium oxysporum sesame specialized pathogenic bacteria are used as transformant, and an exogenous T-DNA sequence is guided into a genome of the fusarium oxysporum sesame specialized strain by combining an appropriate genetic transformation receptor system in an agrobacterium tumefacien-mediated method to obtain the fusarium oxysporum sesame specialized transgenic strain for the first time. The genetic transformation method is high in repeatability and high in conversion rate, and is used for constructing the following fusarium oxysporum sesame specialized mutant library; the obtained strain contains a double-tagging gene, can be used for analyzing the mechanism of sesame fusarium wilt, and is the important innovation in the study of sesame fusarium wilt pathopoiesia and sesame disease resistance.

Description

technical field [0001] The invention relates to a method for exogenous gene transformation of the Fusarium oxysporum sesame-specialized strain mediated by Agrobacterium, and belongs to the field of biotechnology. Background technique [0002] Sesame Fusarium Wilt is one of the main diseases of sesame, which is mainly distributed in the sesame cultivation areas in the north and middle of my country, and it is common and serious. The disease is caused by the infection of Fusarium oxysporum specialized strain of sesame. In the absence of a host, the pathogenic chlamydospores can survive in the soil for more than 6 years, and single methods such as chemical control, physical control and biological control cannot treat the disease. The disease has been well prevented and controlled. At present, people's understanding of the pathogenic mechanism of Fusarium oxysporum sesame-specific type is not deep enough, and the research progress is slow. In the study of the pathogenicity of F...

AI Technical Summary

Problems solved by technology

The disease is caused by the infection of the sesame-specific strain of Fusarium oxysporum. In the absence of a host, the pathogenic chlamydospores can survive in the soil for more than 6 years. The single use of chemical control, physical control and biological control cannot control the disease. The disease has been better prevented and controlled. At present, people's understanding of the pathogenic mechanism of Fusarium oxysporum sesame-specific type is not deep enough, and the research progress is slow.

Images