Preparation method for total saponins of Rubus parviflolius

The technology of total saponins and cogongrass, which is applied in the field of preparation of traditional Chinese medicine extracts, can solve the problems of unfavorable preparation molding, affecting the appearance of preparations, and containing many impurities, and achieves the effects of improving the appearance of preparations, increasing the content of total saponins, and having good repeatability.

Active Publication Date: 2012-06-13
ZEIN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the basis of the above method, the inventors optimized the extraction process and the macroporous adsorption resin purification process of the total saponins of chrysanthemum berries, and found that the highest content of total saponins in the total saponins of chrysanthemum berries could only reach 67.4%, containing There are more impurities and darker color, which is not conducive to the formulation of the preparation and affects the appearance of the preparation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation of embodiment 1, the total saponins of Maoberry

[0018] a. Take the medicinal material of mao berry, crush it into a coarse powder, and extract it 3 times by ultrasonic. For the first time, add 10 times the mass percentage concentration of the medicinal material and a sodium bicarbonate solution with a mass percentage concentration of 0.08%, and then add 8 times the mass percentage concentration of the medicinal material for the second time. 0.05% sodium bicarbonate solution, add 8 times the volume of medicinal material for the third extraction, 1 hour each time, combine three extracts, concentrate to 2 times the volume of medicinal material, add ethanol until the alcohol content is 75 %, stand still for 24 hours, filter, and recover ethanol from the filtrate under reduced pressure until the alcohol content is 20%, adjust the pH to 6 with hydrochloric acid, place at 10°C for 24 hours, filter, and collect the precipitate;

[0019] b. The precipitate o...

Embodiment 2

[0020] The preparation of embodiment 2, the total saponins of Maoberry

[0021] a. Take mao berry medicinal material, crush it into a coarse powder, decoct and extract 3 times, add 10 times the mass percentage of the medicinal material for the first time and a sodium bicarbonate solution with a concentration of 0.07%, and add 8 times the mass percentage of the medicinal material for the second time Sodium bicarbonate solution with a concentration of 0.03%, add 8 times the amount of medicinal material for the third time to extract once, each time for 1 hour, combine three extracts, concentrate to 2 times the volume of the medicinal material, add ethanol until the alcohol content is 70%, stand still for 24 hours, filter, and recover ethanol from the filtrate under reduced pressure until the alcohol content is 15%, adjust the pH to 5 with hydrochloric acid, place at 10°C for 24 hours, filter, and collect the precipitate;

[0022] b. The precipitate obtained in step a is disso...

Embodiment 3

[0023] The preparation of embodiment 3, the total saponins of Maoberry

[0024] a. Take mao berry medicinal material, crush it into a coarse powder, decoct and extract 3 times, add 8 times the mass percentage of the medicinal material for the first time and a sodium bicarbonate solution with a concentration of 0.09%, and add 6 times the mass percentage of the medicinal material for the second time Sodium bicarbonate solution with a concentration of 0.05%, add 6 times the amount of medicinal material for the third extraction with water, 1 hour each time, combine three extracts, concentrate to 2 times the volume of the medicinal material, add ethanol until the alcohol content is 70%, stand still for 24 hours, filter, and recover ethanol from the filtrate under reduced pressure until the alcohol content is 18%, adjust the pH to 6 with hydrochloric acid, place at 10°C for 24 hours, filter, and collect the precipitate;

[0025] b. The precipitate obtained in step a is dissolved...

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Abstract

The invention discloses a preparation method for total saponins of Rubus parviflolius. The method comprises the following steps: extracting the medicinal material Rubus parviflolius with alkaline water at first and then with water, condensing an obtained extract, carrying out alcohol precipitation, condensing the extract again, adjusting the pH value of the extract to be 5 to 6, allowing the extract to stand, filtering the extract, dissolving an obtained precipitate with alkaline water, allowing an obtained solution to pass through a macroporous alkaline anion exchange resin, carrying out flushing sequentially with alkaline water and water, discarding flushing liquor, carrying out elution by using hydrous ethanol, condensing an obtained eluate, allowing the eluate and filtering the eluateto stand so as to obtain a precipitate which is the total saponins of Rubus parviflolius. Compared to the prior art, the method provided in the invention enables the content of total saponins in the total saponins of Rubus parviflolius to be substantially increased; a prepared product has few impurities and a good color, which is favorable for formation of a preparation and improvement of appearance of the preparation; in addition, the method has the advantages of simple operation, good repeatability, stable product quality and suitability for industrial large scale production.

Description

technical field [0001] The invention belongs to traditional Chinese medicine extraction technology, and relates to a preparation method of traditional Chinese medicine extract. Background technique [0002] The traditional Chinese medicine Maoberry is a commonly used Chinese herbal medicine in the folk, which is derived from the dried whole herb of Rubus parvifolius L., a plant of the genus Rubus in the family Rosaceae. It is cool in nature and bitter in taste, and its roots, stems, and leaves can be used as medicine. It has the effects of clearing heat and detoxifying, cooling blood to stop bleeding, expelling wind and dampness, killing insects and treating sores, etc. Metrorrhagia, traumatic bleeding, scabies and other diseases. [0003] CN200310111034.X discloses that the total saponins of chrysanthemum berries have a good therapeutic effect on diseases of the cerebrovascular system such as cerebral ischemia, cerebral thrombosis, and cerebral infarction. -80% ethanol fo...

Claims

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Application Information

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IPC IPC(8): A61K36/73A61P9/10A61P7/02
Inventor 陈剑鸿夏培元王显凤胡小刚张宏伟黄明春
Owner ZEIN BIOTECHNOLOGY CO LTD
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