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Platycodon grandiflorum polysaccharide, and degradation product, preparation method and application thereof

A technology of platycodon polysaccharides and degradation products, which is applied in the direction of sugar derivatives, sugar production, sugar derivatives, etc., and can solve the problems of anti-tumor drug resistance and other problems

Active Publication Date: 2014-09-10
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, clinical first-line anti-angiogenesis anti-tumor drugs have gradually developed drug resistance

Method used

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  • Platycodon grandiflorum polysaccharide, and degradation product, preparation method and application thereof
  • Platycodon grandiflorum polysaccharide, and degradation product, preparation method and application thereof
  • Platycodon grandiflorum polysaccharide, and degradation product, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Extracting platycodon polysaccharides from platycodon rhizome

[0031] Take 3000 g of dried platycodon root, degrease 2 times (5 days / time) with 15 L of 95 wt % ethanol, filter and then dry naturally at room temperature. The dried bellflower decoction pieces were extracted with boiling water, adding 30 L of water each time for 6 hours, and extracted 4 to 6 times in total. After extraction, filtered to obtain the extract, the sulfuric acid-phenol method was used to detect the sugar content in the extract until the sugar reaction was not obvious. The extracts were combined, concentrated to about 10L, deproteinized with 15% (w / v) trichloroacetic acid, centrifuged, and the resulting supernatant was neutralized to pH 7.0 with 10% NaOH, and then dialyzed against flowing water for 48 hours, the inner liquid Concentrate to 1-2L, add three times the volume of 95% ethanol under stirring, let it stand overnight, and centrifuge, the obtained precipitate is washed with abs...

Embodiment 2

[0040] Example 2 Preparation of Platycodon grandiflora polysaccharide degradation product

[0041] Take 250mg of Platycodon grandiflora pectin polysaccharide and place it in a dry 150ml round bottom flask, add 50ml of 0.05mol / L trifluoroacetic acid, seal it tightly, and incubate at 100°C for 1h. After the reaction liquid was distilled off under reduced pressure to remove trifluoroacetic acid, it was dialyzed against distilled water (500ml×4) for 48h, and the inner and outer liquids of the dialysis bag were respectively concentrated and then freeze-dried. The inner solution was lyophilized to obtain secondary platycodon polysaccharide 4-3bde (98mg). Combine the lyophilized products of the two dialyzed external fluids, separate them with Bio-Gel P-2 gel filtration column, detect them with sulfuric acid-phenol method, use standard sugar as a control, collect the components according to the peak position and freeze-dry them to obtain four components , respectively component 1 (4-...

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Abstract

The invention relates to platycodon grandiflorum polysaccharide extracted from rhizome of platycodon grandiflorum, a degradation product of the polysaccharide, a method for extracting the platycodon grandiflorum polysaccharide from the rhizome of the platycodon grandiflorum, a method for preparing the degradation product of the platycodon grandiflorum polysaccharide, and application of the platycodon grandiflorum polysaccharide and the degradation product thereof to preparing a medicine for treating anti-tumour cell angiogenesis diseases. The structure of the platycodon grandiflorum polysaccharide disclosed by the invention is (1->4)-alpha-D- pyranose homogalacturonan, has the polymerization degree of 15-100, the corresponding molecular weight of 2.0-18.0 kD, and the specific rotation of [alpha]D19+125 DEG (c0.05, H2O), and is represented by the following structural formula described in the specification. The molecular weight range of a degradation product obtained by partial acid hydrolysis of the platycodon grandiflorum pectin polysaccharide is 1.4-3.0 kDa. Vivo experiments prove that the platycodon grandiflorum polysaccharide can obviously inhibit lumen generation function of a human microvascular endothelial cell (HMEC-1) and is hopefully used as a novel anti-tumour medicine for inhibiting the growth of a solid tumour by inhibiting angiogenesis.

Description

technical field [0001] The present invention relates to a platycodon polysaccharide extracted from platycodon root, a degradation product of the polysaccharide, a method for extracting platycodon polysaccharide from platycodon root and a preparation method of platycodon polysaccharide degradation product, the present invention also relates to platycodon polysaccharide and its degradation product in Application in the preparation of medicines for treating diseases of anti-tumor cell angiogenesis. Background technique [0002] Neovascularization is the process of generating new blood vessels from existing blood vessels, such as capillaries and postcapillary venules, which is closely related to many physiological and pathological changes. Regulation, and its abnormality leads to related diseases. The concept that tumor growth is dependent on angiogenesis began in the early 1970s and was first articulated by Judah Fokman. In the past ten years, due to the discovery of the role...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C07H7/033A61K31/715A61K31/702A61K31/7012A61P35/00C13K13/00C13K5/00
Inventor 丁侃董群徐玉霞邱宏
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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