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Method for extracting 5-hydroxytryptophan

A technology of hydroxytryptophan and extract, which is applied in the direction of organic chemistry, can solve the problems of high production cost and low yield, and achieve the effects of low cost, increased product yield, and shortened production cycle

Inactive Publication Date: 2012-05-23
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As mentioned above, the existing technology has the disadvantages of high production cost and low yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Ghana seed raw material is crushed to 20-40 mesh, take 2kg, add 30kgpH3 hydrochloric acid aqueous solution and 30g sodium metabisulfite, stir and soak for 10 hours, extract the supernatant, add 16kg hydrochloric acid water for 4 hours, filter and combine the extract, add 3LHD- 8. After adsorption by cation exchange resin column, wash with deionized water until sugar-free color, then elute with 18L of 10% ammonia solution (containing 1‰ sodium pyrosulfite), collect the eluent hydrochloric acid to adjust to pH2, and then add 2LHZ818 macroporous resin column for adsorption , washed with water until the resin column is neutral, and 12L of 40% ethanol solution elutes the active ingredient to obtain an alcohol eluent. The eluate was decolorized by adding 120 g of activated carbon to reflux, the activated carbon was filtered off, the decolorized solution was concentrated to a density of 1.5, left to crystallize, the crystals were filtered off, and vacuum-dried to obtain white 5...

Embodiment 2

[0026] Ghana seed raw materials are crushed to 20-40 mesh, take 2kg, add 20kgpH2 hydrochloric acid aqueous solution and 40g sodium metabisulfite, stir and soak for 6 hours, extract the supernatant, add 20kg hydrochloric acid water for 6 hours, filter and combine the extract, add 4L003× 7 After the cation exchange resin column is adsorbed, wash it with deionized water until it has no sugar color, and then elute it with 16L of 15% ammonia solution (containing 3‰ sodium metabisulfite), collect the eluate and adjust the pH to 3 with hydrochloric acid, then add 2LAB-8 macroporous resin Column adsorption, washed with water until the resin column is neutral, 8L of 60% ethanol solution to elute the active ingredient to obtain alcohol eluent. The eluate was decolorized by adding 100 g of activated carbon to reflux, filtered off the activated carbon, concentrated the decolorized solution to a density of 1.6, placed it to crystallize, filtered off the crystal, and dried in vacuum to obtai...

Embodiment 3

[0028] Ghana seed raw material is crushed to 20-40 meshes, 5 kg is added to 50 kg pH3 hydrochloric acid aqueous solution and 50 g sodium metabisulfite, stirred and soaked for extraction for 8 hours, the supernatant is extracted, and then 50 kg of hydrochloric acid water is soaked for 4 hours to extract twice, and the combined extract is filtered. After adding 8LHD-8 cation exchange resin column for adsorption, wash with deionized water until sugar-free color, then elute with 40L of 12% ammonia solution (containing 2‰ sodium metabisulfite), collect the eluent hydrochloric acid to adjust to pH4, then add 6L of LSA- 20 macroporous resin column for adsorption, washed with water until the resin column is neutral, 30L of 50% ethanol solution to elute the active ingredient to obtain ethanol eluent. The eluate was decolorized by adding 300 g of activated carbon under reflux, filtered out the activated carbon, concentrated the decolorized solution to a density of 1.4, placed it to cryst...

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PUM

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Abstract

The invention relates to a method for extracting 5-hydroxytryptophan. The method has the advantages that: the yield of the product is improved and the energy consumption is reduced because an antioxidant is added to protect and an ion exchange resin and a macroporous resin are combined. The method comprises the following steps of: crushing a raw material, namely griffonia seeds, soaking the raw material into acidic water and sodium pyrosulfite for extraction for 2 to 3 times, wherein the amount of the acidic water and the amount of the sodium pyrosulfite are both 8 to 15 times that of the raw material and the raw material is soaked for 4 to 10 hours at each time, adsorbing the extract by adding the extract into a cation exchange resin column, washing the extract with deionized water until the extract is colorless, eluting the extract with aqueous ammonia (containing 0.1 to 0.3 percent of sodium metabisulfite) of which the concentration is 10 to 15 percent, adjusting the pH value of the eluate to 2 to 4, adsorbing the eluate by adding the eluate into a macroporous resin column, washing the eluate with water until the eluate is neutral, eluting the effective component with 4-6 BV ethanol solution of which the concentration is 40 to 60 percent, collecting the eluate, adding the eluate into active carbon to decolor the eluate, reclaiming the ethanol, concentrating the decoloring liquid until the concentration of the decoloring liquid is 1.4 to 1.6, placing the decoloring liquid until the decoloring liquid is crystallized, filtering the crystal, and drying the crystal to obtain the product. The method is simply operated and is suitable for industrial production.

Description

Technical field: [0001] The invention belongs to the field of separation of natural products, in particular to a method for extracting 5-hydroxytryptophan. Background technique: [0002] 5-Hydroxytryptophan, English name is 5-hydroxytryptopHan, chemical name: 5-hydroxy-3-indolyl-alanine, molecular formula: C 11 h 12 N 2 o 3 , molecular weight: 220.23. [0003] 5-Hydroxytryptophan is a precursor substance of a hormone called serotonin in the human body, which can help the production of this hormone in the human body. 5-Hydroxytryptophan is an important inhibitor of neurotransmitter serotonin (5-HT ) precursor. There are serotonin in the brain, platelets, stomach, and central nervous system. When the mood changes, serotonin will be secreted accordingly. It is an important chemical substance in the brain to control emotions, behaviors, appetite, sleep, and impulse. 5-hydroxytryptamine can effectively help produce serotonin, so it can promote the health of mood and nervous...

Claims

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Application Information

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IPC IPC(8): C07D209/20
Inventor 李法庆杨成东刘东锋
Owner 苏州宝泽堂医药科技有限公司
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