Eupolyphaga sinensis Walker activity polypeptide soy and preparation method thereof

Abstract

The invention relates to a preparation method of a Eupolyphaga sinensis Walker activity polypeptide soy. The preparation method is characterized in that the preparation method comprises the following steps: 1, selecting Eupolyphaga sinensis Walker with the protein content of greater than 60% (by dry weight); 2, roasting and crushing it; 3, adding distilled water to it according to a material / water ratio of 1:5-1:50 to dissolve it, adjusting the pH value of the obtained solution to 8.2 by a 2.5mol / L Na2CO3 solution, rapidly adding 50-1000U of an Arazyme protease, well shaking, and carrying out enzymatic hydrolysis for 1-6h in a constant temperature water bath oscillator under conditions that the temperature is 37DEG C and the rotation speed is 150r / min; and 4, carrying out enzyme inactivation on the obtained enzymatic hydrolysate, adding the enzymatic hydrolysate to a low-salt solid soy (prepared according to a BNJL-016-2003 process) in the early fermentation stage, the middle fermentation stage and the late fermentation stage, and fermenting (according to the BNJL-016-2003 process) to obtain the novel insect activity polypeptide soy with the polypeptide content of greater than 100mg / ml and the antioxidation activity (represented by the DPPH free radical clearance) of not lower than 65%. The preparation method of the soy of the invention, which treats soybean meal, wheat bran and the like as main materials and a high protein insect Eupolyphaga sinensis Walker as an auxiliary material, adopts the efficient proteolytic enzyme Arazyme (the Arazyme is a high-activity metal proteolytic enzyme secreted by a symbiotic microbe (HY-3) in the alimentary canal of Nephila Clavata) and combines a traditional soy fermentation technology with an enzyme technology, allows the Eupolyphaga sinensis Walker polypeptide soy with the antioxidation activity to be prepared.

Description

technical field [0001] The invention relates to insect polypeptide soy sauce and a preparation method thereof. More specifically, it relates to a method for preparing low-salt solid soy sauce with anti-oxidation activity by using the soft-shelled soft-shelled turtle medicinal insect combined with enzyme technology and fermentation technology. Background technique [0002] Eupolyphaga sinensis Walker is also known as Eupolyphaga sinensis Walker, Eupolyphaga sinensis Walker, etc. Its medicinal name is Su Tuyuan. It is a traditional and valuable traditional Chinese medicinal material with a history of more than 2,000 years. Bone pain, swelling, blowing throat, etc. In recent years, a large number of modern medical experimental studies and clinical observations have confirmed that the ground beetle has the functions of anti-cancer, lowering blood fat, tolerance to hypoxia, improving immunity, and health care for liver cirrhosis, brain injury, vasculitis, osteomyelitis, and bloo...

AI Technical Summary

Problems solved by technology

But these varieties of soy sauce are far from meeting the consumption requirements of different levels in modern society.