Primers for amplifying Rrspgl, core fragment of gene and application of core fragment

Abstract

The invention discloses primers for amplifying Rrspgl, a core fragment of the gene and application of the core fragment. Nucleotide sequences of the specific primers for amplifying the Rrspgl gene are shown as SEQ ID NO:1 and SEQ ID NO:2; specific primers for amplifying the core fragment of the gene are shown as SEQ ID NO:3 and SEQ ID NO:4; and a nucleotide sequence of the core fragment of the gene is shown as SEQ ID NO:5. In the invention, a ribonucleic acid interference (RNAi) expression vector pSilent-PG-2 of the core fragment of the Rrspgl is also constructed; and the RNAi expression vector is obtained by performing double digestion on a vector pSilent-1 and the core fragment of the Rrspgl through two groups of restriction enzymes, namely HindIII and XhoI as well as KpnI and BglII respectively, and then performing forward and reverse connection. The function of the core fragment of the Rrspgl is simultaneously researched, so that a great guiding significance is provided for the research such as the disclosure of the pathogenic mechanism of rhizoctonia solani and the molecular mechanism of the interaction between the rhizoctonia solani and hosts and the like.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to amplification Rrspg1 (Rice sheath blight endopolygalacturonase gene 1) specific primers, specific primers for amplifying the core fragment of the gene, the core fragment of the gene and its application. Background technique [0002] Rice sheath blight is one of the three major diseases of rice worldwide, which has caused serious economic losses in our country. The causative agent of rice sheath blight is Rhizoctonia solani ( Rhizoctonia solani Kühn), which has a wide distribution range and host range, and can damage more than 260 kinds of plants such as corn, soybean and potato in addition to rice. Due to Rhizoctonia solani ( R. solani ) species members are complex, so the mycelium fusion group (anastomosis group, AG) is usually used for reclassification. Currently Rhizoctonia solani ( R. solani ) are divided into at least 14 AGs, and some AGs are further divi...

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There are no patent reports on the specific amplification primers for endopolygalacturonase gene of rice sheath blight, the specific primers for amplifying the core fragment of the gene, the core fragment of the gene and its application.

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