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A kind of Moutan cortex extract and its preparation method and application

A technique for extracting and using Cortex Moutan, applied in the field of traditional Chinese medicine, can solve problems such as undiscovered, affecting virus secretion, etc.

Active Publication Date: 2015-09-09
云南白药中草药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, there is no report on the effect of Moutan cortex extract on GRP78 to affect virus secretion

Method used

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  • A kind of Moutan cortex extract and its preparation method and application
  • A kind of Moutan cortex extract and its preparation method and application
  • A kind of Moutan cortex extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1. Application 50% ethanol-water solution prepares Moutan cortex extract

[0046] The preparation method of the Cortex Moutan extract of embodiment 1 is as figure 1 shown in the flow chart. Specifically, add 10 kg of dried root bark of Paeonia suffruticosa Andr. to 100 kg of 50% ethanol-water solution at room temperature, stir and soak for 8 hours, then filter out the extract, and add 80 kg of 50% ethanol to the filter residue at room temperature In the ethanol-water solution, stir and soak for 8 hours, filter the extract, add the filter residue to 60kg of 50% ethanol-water solution at room temperature, stir and soak for 8 hours, combine the three extracts and concentrate under reduced pressure to 1 / 30 volume, and the concentrated solution reduces Press drying or vacuum freeze-drying to obtain the Moutan cortex extract.

Embodiment 2

[0047] Embodiment 2. The ethanol aqueous solution of application different concentration (30%, 40%, 50%, 60%, 70%) prepares Cortex Moutan extract

[0048] refer to figure 1 Flow chart shown, preparation of Cortex Moutan extract. Specifically, 5 parts of Cortex Moutan (1 kg each) was added into ethanol aqueous solution (10 kg) of corresponding concentration (30%, 40%, 50%, 60% or 70%) at room temperature and stirred and soaked for 8 hours. Afterwards, the first filtrate and the first filter residue are separated by filtration. The first filter residue was added into ethanol aqueous solution (8 kg) of corresponding concentration (30%, 40%, 50%, 60% or 70%) at room temperature and stirred and soaked for 8 hours. Subsequent filtration separates the second filtrate and the second filter residue. The second filter residue was added into ethanol aqueous solution (6 kg) of corresponding concentration (30%, 40%, 50%, 60% or 70%) at room temperature and stirred and soaked for 8 hour...

Embodiment 3

[0049] Example 3. Utilize herbal medicine chips to screen active ingredients

[0050] The Cortex Moutan extract obtained in Example 2 was screened using the high-throughput screening method for active ingredients in herbal medicines described in Chinese patent ZL01121114.8. The specific description is as follows (screening results are shown in Figure 4 ):

[0051] ①Classification of herbal extracts by HPLC

[0052] In Example 2, use five different concentrations of ethanol aqueous solution to extract the Cortex Moutan extract, respectively take an appropriate amount of the resulting freeze-dried powder and dissolve it in 50% ethanol aqueous solution (1ml) to obtain the equivalent of 1g crude drug / ml The solution. The obtained solution was centrifuged at 12000 rpm (Shanghai Anting TGL-16B) for 20 minutes, and the clear supernatant was collected. The clear supernatant was diluted twice with 50% ethanol-water solution and filtered through a 0.45 μm syringe filter. The filtr...

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Abstract

The invention provides a tree peony root bark, as well as a preparation method and application thereof. The preparation method comprises the following steps: extracting a tree peony root bark medicinal material with organic alcohol aqueous solution, concentrating and drying to obtain the tree peony root bark extract. The tree peony root bark extract can inhibit glucose-regulated protein GRP78, and is used for resisting virus. In-vitro experiments prove that: the tree peony root bark extract has remarkable effect in resisting hepatitis B virus secretion, has small toxicity to normal cells and liver cancer cells, and has excellent application prospect.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to a traditional Chinese medicine extract and its preparation method and application, in particular to an extract of Cortex Moutan and its preparation method, and the extract can be used for antiviral. Background technique [0002] Cortex Moutan, also known as Cortex Moutan and Cortex Fen, is the dry root bark of Paeonia suffruticosa Andr. It was first recorded in "Shen Nong's Materia Medica", and is now included in "Chinese Pharmacopoeia". It is slightly cold in nature, bitter and pungent, and returns to the heart, liver and kidney. It has the effects of clearing heat and detoxifying, promoting blood circulation and removing blood stasis. It is used for the treatment of warm toxins and spots, vomiting blood and epistaxis, night heat and early cool, no sweat bone steam, amenorrhea dysmenorrhea, carbuncle sores, bruises, etc. [0003] The main chemical compon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/71A61P31/14A61P31/20A61P31/22A61K125/00
CPCY02A50/30
Inventor 杨晓源黎耀基张大兹黄敏
Owner 云南白药中草药科技有限公司
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