A kind of Moutan cortex extract and its preparation method and application
A technique for extracting and using Cortex Moutan, applied in the field of traditional Chinese medicine, can solve problems such as undiscovered, affecting virus secretion, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0045] Embodiment 1. Application 50% ethanol-water solution prepares Moutan cortex extract
[0046] The preparation method of the Cortex Moutan extract of embodiment 1 is as figure 1 shown in the flow chart. Specifically, add 10 kg of dried root bark of Paeonia suffruticosa Andr. to 100 kg of 50% ethanol-water solution at room temperature, stir and soak for 8 hours, then filter out the extract, and add 80 kg of 50% ethanol to the filter residue at room temperature In the ethanol-water solution, stir and soak for 8 hours, filter the extract, add the filter residue to 60kg of 50% ethanol-water solution at room temperature, stir and soak for 8 hours, combine the three extracts and concentrate under reduced pressure to 1 / 30 volume, and the concentrated solution reduces Press drying or vacuum freeze-drying to obtain the Moutan cortex extract.
Embodiment 2
[0047] Embodiment 2. The ethanol aqueous solution of application different concentration (30%, 40%, 50%, 60%, 70%) prepares Cortex Moutan extract
[0048] refer to figure 1 Flow chart shown, preparation of Cortex Moutan extract. Specifically, 5 parts of Cortex Moutan (1 kg each) was added into ethanol aqueous solution (10 kg) of corresponding concentration (30%, 40%, 50%, 60% or 70%) at room temperature and stirred and soaked for 8 hours. Afterwards, the first filtrate and the first filter residue are separated by filtration. The first filter residue was added into ethanol aqueous solution (8 kg) of corresponding concentration (30%, 40%, 50%, 60% or 70%) at room temperature and stirred and soaked for 8 hours. Subsequent filtration separates the second filtrate and the second filter residue. The second filter residue was added into ethanol aqueous solution (6 kg) of corresponding concentration (30%, 40%, 50%, 60% or 70%) at room temperature and stirred and soaked for 8 hour...
Embodiment 3
[0049] Example 3. Utilize herbal medicine chips to screen active ingredients
[0050] The Cortex Moutan extract obtained in Example 2 was screened using the high-throughput screening method for active ingredients in herbal medicines described in Chinese patent ZL01121114.8. The specific description is as follows (screening results are shown in Figure 4 ):
[0051] ①Classification of herbal extracts by HPLC
[0052] In Example 2, use five different concentrations of ethanol aqueous solution to extract the Cortex Moutan extract, respectively take an appropriate amount of the resulting freeze-dried powder and dissolve it in 50% ethanol aqueous solution (1ml) to obtain the equivalent of 1g crude drug / ml The solution. The obtained solution was centrifuged at 12000 rpm (Shanghai Anting TGL-16B) for 20 minutes, and the clear supernatant was collected. The clear supernatant was diluted twice with 50% ethanol-water solution and filtered through a 0.45 μm syringe filter. The filtr...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap