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Dry chemical quantitative test strip with interference removal, alanine transaminase or aspartate transaminase quantitative test strip and test method

A technology of aspartate transaminase and dry chemistry, which is applied in the field of dry chemical test strips and detection of transaminase, and can solve the problems of prone to false positive results, etc.

Active Publication Date: 2012-04-18
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using the rate method (that is, testing the change rate of color development), removing endogenous interference requires more complicated optical instruments, often using optical devices such as integrating spheres, and the normal value of transaminase is generally very low, and alanine aminotransferase is 5U / L ~40U / L, aspartate aminotransferase is 8U / L~40U / L, and the normal reference value of pyruvate is relatively high, which is 65μmol / L (equivalent to the pyruvate produced by 65U / L transaminase in 1min). During the dry chemical test time (2min~3min), the color change rate of the chromogenic agent will also be affected by endogenous pyruvate to a certain extent, which is prone to false positive results

Method used

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  • Dry chemical quantitative test strip with interference removal, alanine transaminase or aspartate transaminase quantitative test strip and test method
  • Dry chemical quantitative test strip with interference removal, alanine transaminase or aspartate transaminase quantitative test strip and test method
  • Dry chemical quantitative test strip with interference removal, alanine transaminase or aspartate transaminase quantitative test strip and test method

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Embodiment 1

[0055] a kind of like figure 2 The shown dry chemical quantitative test strip for removing interference, the test strip comprises a substrate 7, on which a sample application area 13 fixed with an interference-removing reagent (in this embodiment specifically comprising a diffusion layer 1, a sample Pad 2, blood filtration membrane 3, enzyme binding pad 4 and flow pad 5), the sample application area 13 is directly fixed on the substrate 7; the substrate 7 is also provided with a fixed starting area of ​​the test reaction initiation reagent and signal providing reagent 14. The activation area 14 includes a reagent layer 6, the reagent layer 6 is connected to one end of the substrate 7 through an adhesive 8, and the adhesive 8 has a sufficient height to allow most of the reagent layer 6 to be suspended in the sample loading area. Zone 13 above. The sample application area 13 and the activation area 14 remain non-contact under normal conditions, and the activation area 14 (reagen...

Embodiment 2

[0059] a kind of like image 3 The shown dry chemical quantitative test strip for removing interference, the test strip comprises a substrate 7, on which a sample application area 13 fixed with an interference-removing reagent (in this embodiment specifically comprising a diffusion layer 1, a sample Pad 2, blood filtration membrane 3, enzyme binding pad 4 and flow pad 5), the sample application area 13 is directly fixed on the substrate 7; the substrate 7 is also provided with a fixed starting area of ​​the test reaction initiation reagent and signal providing reagent 14. The activation area 14 includes a reagent layer 6, the reagent layer 6 is connected to one end of the substrate 7 through an adhesive 8, and the adhesive 8 has a sufficient height to allow most of the reagent layer 6 to be suspended in the sample loading area. Zone 13 above. The sample application area 13 and the activation area 14 remain non-contact under normal conditions, and the activation area 14 (reage...

Embodiment 3

[0063] a kind of like Figure 4 Compared with the test strip provided in Example 1, the shown dry chemical quantitative test strip for removing interference does not have an enzyme binding pad 4, and the rest of the structure and application methods are the same as in Example 1 . Since the enzyme-binding pad 4 is not provided, substances originally immobilized on the enzyme-binding pad 4 (such as various catalytic reaction enzymes) can be directly immobilized on other structures in the sample loading area 13 (such as the flow pad 5 ).

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Abstract

The invention discloses a dry chemical quantitative test strip with interference removal, comprising a substrate, a sampling region which is provided with a fixed interference removing reagent and arranged on the substrate, and a promoter region which is provided with a fixed test reaction promoter and a fixed signal supplying reagent and is arranged on the substrate, wherein, the sampling region and the promoter region are distributed on the test strip under the conditions that: the sampling region keeps non-contact with the promoter region in normal state, and the sampling region contacts with the promoter region in test state. The test method comprises the following steps: adding a sample liquid to the tested in the sampling region, redissolving the substance fixed on the sampling region in the sample liquid to the tested, carrying out reaction for removing interference; then contacting the promoter region with the sampling region, redissolving the substance fixed on the promoter region, and carrying out reaction for test; carrying out signal reaction on the reaction product, and according to the tested signal value, calculating the content of biological enzyme to be tested. The test method has the advantages of simple operation, low test cost, accurate test result, and high test precision, and can be concretely applied for the quantitative test of alanine transaminase or aspartate transaminase.

Description

technical field [0001] The invention relates to a dry chemical test strip and a test method, in particular to a dry chemical test strip and a detection method for transaminase. Background technique [0002] There are many interferences in dry chemical tests, such as the interference of uric acid and ascorbic acid in the determination of redox reaction, the interference of pyruvate in the detection of transaminase, especially the interference of pyruvate in transaminase is more serious. [0003] There are many kinds of transaminases, among which alanine aminotransferase (GPT / ALT) and aspartate aminotransferase (GOT / AST) are the most important. Alanine aminotransferase, also known as alanine aminotransferase, catalyzes the transamination between glutamic acid and pyruvate; of transamination. Clinically, the determination of these two transaminases in whole blood, serum, plasma, and interstitial fluid is a very important indicator in the diagnosis of heart disease, muscle dis...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N21/78
Inventor 吉翔谢光李宗祥车宏莉
Owner SINOCARE
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