Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primer, probe and detection kit for determining beta-tubulin III gene expression

A tubulin and gene expression technology, which is used in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. , the effect of simple calculation

Active Publication Date: 2012-04-11
上海厦维医学检验实验室有限公司
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is still no major progress in this field of technology

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer, probe and detection kit for determining beta-tubulin III gene expression
  • Primer, probe and detection kit for determining beta-tubulin III gene expression
  • Primer, probe and detection kit for determining beta-tubulin III gene expression

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Source of sample tissue: 65 tissues from cancer patients who had not undergone chemotherapy were sent to our company for testing in 2010-2011. Among the 65 patients, there were 39 males and 21 females, including 54 adenocarcinomas and 11 squamous cell carcinomas.

[0044]Primer design and synthesis: Primers were designed according to the GeneBank sequence β-tubulin Ⅲ and the housekeeping gene β-actin. Primers and probes were designed using the Premier 5.0 software of Canada's Premier Company, and the sequences of the primers and probes are shown in Table 1.

[0045] Pathological tissue RNA preparation: For fresh pathological tissue, about 1 g was cut in a liquid nitrogen environment, mashed and RNA was extracted using an RNA extraction kit (Qiagen, Blood & Tissue Kit). The specific steps follow the operating instructions of the kit. For paraffin-embedded tissue samples, RNA was extracted using an RNA extraction kit (Qiagen, RNeasy FFPE kit), and the specific steps were...

Embodiment 2

[0061] Two cases of paraffin-embedded tissue samples from patients with adenocarcinoma who were sent to our company for detection in May 2011 were recorded as patient A and patient B. According to the detection kit and method described in Example 1, β-tubulin III mRNA was extracted, cDNA was synthesized, and real-time fluorescent PCR quantitative assay was carried out (the experimental steps were operated according to the operating steps of the kit instructions). Four gradient standards of β-tubulin Ⅲ cDNA and housekeeping gene β-actin were used as standard curves respectively, and the correlation coefficient between β-tubulin Ⅲ and housekeeping gene β-actin was 0.98. Record the value C1 of the test sample on the standard curve of the β-tubulin III gene, and the value C2 of the test sample on the standard curve of the internal reference gene β-actin.

[0062] Use the formula: Y=ln(C1 / C2)

[0063] The β-tubulin Ⅲ gene expression levels of the two patients were calculated respe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a primer, probe and detection kit for determining beta-tubulin III expression. The primers and probes for determining beta-tubulin III gene expression include TUBB3-F TCTACTACAACGAGGCCTCTTCT, TUBB3-R CAAAGATGAAATTGTCAGGCCTGAA, TUBB3-P AGCCACACCAGAATGGCTCGAGGCACGTACCTCG and TUBB3-reverse transcription-TTGCCGGCCCCACTCTGACCAAA. The detection kit can measure the expression level of beta-tubulin III mRNA and provide a method for predicting possible resistance to treatment based on paclitaxels or vinblastines for the subjects.

Description

field of invention [0001] The present invention relates to the field of biotechnology. It specifically relates to the use of quantitative fluorescent PCR to determine the gene expression of β-tubulin III (β-tubulin III) in tumor cells and a primer, probe, kit and detection method for detecting the expression of β-tubulin III . Background of the invention [0002] Tumor is formed when normal cells of the body are exposed to various carcinogenic factors, and a certain cell in the local tissue loses normal regulation of its growth at the gene level, resulting in abnormal clonal proliferation. Malignant tumors, or cancer, form when tumor cells continue to grow and divide at a high rate, grow out of control, invade normal tissues, and often metastasize to sites far from their origin. When tumors form, the clinical goal is to selectively kill tumor cells while reducing damage to normal cells in the body during treatment. [0003] Chemotherapy refers to the use of chemical drugs...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 张海龙阮力施伟杰郑立谋
Owner 上海厦维医学检验实验室有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com