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Brevibacillus laterosporus, microorganism strain agent and microorganism fertilizer prepared by using brevibacillus laterosporus as well as preparation methods thereof

A technology of Bacillus lateralis and microbial strains, which is applied in the field of microbial inoculants, can solve the problems of poor product quality, short shelf life, low survivability of bacterial strains, etc., and achieves short shelf life, high effective bacterial count, and long shelf life. Effect

Inactive Publication Date: 2012-04-04
麻林涛 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems in the prior art, such as low strain viability, substandard number of effective viable bacteria, short shelf life, poor product quality, unstable application effect, etc., one of the purposes of the present invention is to provide a new Bacillus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: Utilize traditional method to carry out fermentation preparation microbial strain agent to No. 1 on the new side

[0050] Medium: 3% starch, 1% bean cake powder, 0.1% peptone, 0.2% ammonium sulfate, 0.2% dipotassium hydrogen phosphate, 0.1% yeast powder, 0.01% calcium carbonate, pH7.2-7.4.

[0051] After activating the strain, put it into 1000ml of the above-mentioned medium, and culture it in the Erlenmeyer shaker flask at 160RPM at 37°C for 16 hours, then inoculate it into a 200L seed fermenter (still the same medium) with 1% inoculum amount, and ventilate at 37°C Cultivate for 16 hours, then transfer to 2000L fermenter according to 10% inoculation amount (still the same culture medium), incubate at 37°C for 20 hours, then raise the temperature to 42°C and cultivate for 10 hours, stop the fermentation after 80% of the bacteria produce spores . Detect the bacterial concentration and reach 10 9 , and then mixed with high-quality peat powder (commercial...

Embodiment 2

[0052] Embodiment 2: Utilize the method provided by the invention to carry out fermentation preparation microbial strain agent to No. 1 on the new side

[0053] Activate the strains first, then rinse the slope with 5ml of sterile water to form a bacterial suspension, and put the bacterial suspension into a 500ml triangular flask containing 100ml of new 1 liquid medium, and use a shaker to cultivate for 18 hours. 37°C, 180-200rpm, detect the bacterial concentration, when the concentration reaches 10 8 Order of magnitude, proceed to the next step;

[0054] Transfer 100ml of bacterial solution from a 500ml conical flask into a 2000ml conical flask, which contains 700ml of new 2 medium, and cultivate it on a shaker for 20 hours at 37°C, 180-200rpm; Concentration up to 10 8 Order of magnitude, proceed to the next step;

[0055] Put the bacterial solution in the 2000ml Erlenmeyer flask into 1m 3 In the fermenter, the intake volume is 6 liters, the fermenter contains 500L of ne...

Embodiment 3

[0057] Embodiment 3: Under the condition that does not stockpile for a long time, carry out bacterial count detection to the microbial strain preparation that utilizes traditional method to prepare

[0058] The live bacteria content in the microbial strain agent was detected by the conventional dilution plate counting method. Weigh 1 gram of microbial strain agent (stocked for half a month), dissolve it with sterile water, dilute it with sterile water until the number of bacteria is 20-100 / ml, and then pipette 1ml of the diluted solution into a sterile petri dish , add an appropriate amount of new medium 1 (slant medium), mix well and solidify, incubate upside down in a 37°C incubator for 48 hours, count the colonies on the plate, repeat more than three times, and multiply the average value by the one gram of microbial strains The number of milliliters when the agent is diluted so that the concentration of bacteria is 20-100 / ml is the number of strains per gram of microbial ...

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Abstract

The invention relates to a novel brevibacillus laterosporus which has the collection number of CGMCC (China General Microbiological Culture Collection Center) No.5090, a microorganism strain agent and a microorganism fertilizer prepared by using the strain as well as preparation methods thereof. According to the strain provided by the invention, the produced microorganism strain agent has the advantages of higher effective microbial content and longer quality guarantee period.

Description

technical field [0001] The present invention relates to a novel Bacillus lateralosporus, microbial strain agent and microbial fertilizer prepared by the strain and their preparation method. Background technique [0002] my country has a large population but little arable land, and the arable land decreases year by year with the growth of population and the development of industry and cities. According to the statistics of relevant departments, the average population of our country increases by 16 million every year, while the cultivated land decreases by 4 million mu every year. In 1950, the population was 550 million, the arable land was 1.65 billion mu, and the per capita arable land was 2.99 mu. In 1980, the per capita arable land was 1.51 mu. In 1990, the per capita arable land dropped to 1.1 mu. Acre level. Therefore, the only feasible way to increase the output of agricultural products in our country is to increase the output per unit area. In order to meet the dema...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G1/00C12R1/07
Inventor 麻林涛王莉
Owner 麻林涛
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