Method for controlling grain pest by using essential oil
A technology of plant essential oils and pests, which is applied in the field of pest control in stored grains, can solve the problems of lowering grain grades, short effective period of insect control, and easy volatilization of plant essential oils, and achieves the effects of broad application prospects, reduced dosage, and reduced drug costs
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Embodiment 1
[0018] Embodiment 1 essential oil and its mixture toxicity test with diatomaceous earth
[0019] With the red-spotted corn beetle as the experimental object, the method of fumigation and poisoning with mixed grain was adopted.
[0020] Take several different amounts of plant essential oils: cinnamon ( Cinnamomum cassia ) essential oil E8, allicin ( Allium sativum ) essential oil E12 and litsea cubeba ( Litsea cubeba ) essential oil E20 (the amount of each essential oil is: 10μl, 15μl, 20μl, 25μl, 30μl and 40μl), directly mix different amounts of essential oil with 50g rice, insert 60 adults, cover all bottles with nylon gauze and plastic wrap Seal the mouth well, and put it into a dark condition with a temperature of 25°C and a humidity of about 75% for observation.
[0021] In the mixed treatment group of essential oil and diatomaceous earth, 12.5mg of diatomaceous earth was first mixed with different amounts of the above three essential oils (0.5μl, 1.5μl, 2.0μl, 2.5μ...
Embodiment 2
[0026] Embodiment 2 essential oil and the mensuration of its mixed action duration with diatomaceous earth
[0027] The treatment method of essential oil and diatomaceous earth is the same as in Example 1 (the amount of essential oil is the amount required for the mortality of test insects to reach about 90% within 24 hours). The concentration of essential oil required for the treatment group is E12 11 μl (1.2 μl required for mixture with 5 mg diatomaceous earth), E8 11 μl (1.2 μl required for mixture with 5 mg diatomaceous earth), E20 13 μl (1.2 μl required for mixture with 5 mg diatomaceous earth) Volume 1.3 μl). After the treatment, seal all the bottle mouths with nylon gauze and plastic wrap, and put them into a dark condition with a temperature of 25°C and a humidity of about 75% for subsequent use. On the third day, put the 30 red beetles that had been starved for 24 hours into 20g of organic rice, and observe the mortality rate of each group after 24 hours (touch the...
Embodiment 3
[0029] Example 3: Realistic heritability and risk assessment of pest resistance
[0030] When estimating the heritability of resistance, we usually consider resistance as a threshold trait with a normal distribution. The mean and variance of the distribution were calculated according to the regression equation of the logarithm of dose-mortality probability value. Specific methods refer to Ding Wei et al. (Ding Wei, Wang Jinjun, Zhao Zhimo, Zhang Yongqiang, Tao Huiying. Breeding of air-resistant and drug-resistant strains of Bookhopper curlyphila and risk analysis of resistance. Chinese Journal of Plant Protection, 2006, 33(1): 81 -86). At the same time, we can also estimate the heritability changes of resistant populations after multiple generations of selection, so as to predict the time when pest resistance appears. According to previous research reports, the formula can be used to calculate the number of generations required for pests to develop 10-fold resistance.
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