Spittle alcoholicity test strip and production method thereof

Abstract

The invention provides a spittle alcoholicity test strip and a production method thereof. The test strip comprises a substrate and a reaction test strip which is adhered to the surface at one side surface of the substrate; and the reaction test strip is dry filter paper which is soaked in a color-developing agent solution and a bi-enzymatic reaction liquid in sequence. The production method comprises the following steps of: soaking the filter paper in the color-developing agent solution and then drying the filter paper by means of being protected from light at a room temperature to obtain developing filter paper; and soaking the developing filter paper into the bi-enzymatic reaction liquid and then drying the developing filter paper by means of being protected from the light at the room temperature to obtain the reaction filter paper. The bi-enzymatic reaction liquid contains alcohol oxidase with the activity unit of 80 U / ml, peroxidase with the activity unit of 80 U / ml, bovine serum albumin which accounts for 1.6% of the total weight of the bi-enzymatic reaction liquid, trehalose which accounts for 1.5% of the total weight of the bi-enzymatic reaction liquid, and the balance of 0.3 M phosphate buffer with the pH value of 7.5. The test strip has the advantages of fastness and accuracy in detection, high sensitivity and good stability. Furthermore, the production method is simple, practical and very reliable.

Description

technical field [0001] The invention relates to a saliva alcohol content test strip, in particular to a saliva alcohol content test strip and a manufacturing method thereof, belonging to the technical field of biosensors. Background technique [0002] In recent years, with the rapid development of the transportation industry, the number of motor vehicles and the number of people with driver's licenses have increased rapidly. At the same time, the number of traffic accidents has also increased. Among them, traffic accidents caused by drivers drinking and drunk Accounting for 20%; Therefore, fast, accurate detection driver's body alcohol concentration is subject to more and more attention. [0003] Alcohol detection plays an important role in forensic science and clinical testing, and the detection of blood alcohol concentration can be used to diagnose acute alcoholism and some syndromes related to alcohol abuse; at present, alcohol content testing techniques mainly include br...

AI Technical Summary

Problems solved by technology

[0005] Existing alcohol test strips are easily affected by temperature and humidity, which leads to limited storage conditions and storage time of test strips. Temperature and humidity are one of the important factors affecting the efficiency and storage of enzyme reactions, which is the cause of enzymatic reaction reagents. Reasons that cannot be preserved for a long time; the enzyme activation components adopted in the existing alcohol test strips are toxic, harmful to the human body, polluting the environment, and cannot effectively balance the activity and stability of the enzyme; in addition, the test strips made by the prior art , there are some abnormal background colors, which can easily lead to large errors in testing and comparison

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