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Method for extracting polysaccharide from imperata rhizome

A technology of Imperata corydalis and polysaccharides, applied in the biological field, can solve the problems of extraction rate and extraction method research, and does not involve purification methods, etc., and achieve the effects of shortening extraction time, less pollution, and lower energy consumption

Inactive Publication Date: 2012-03-21
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the extraction rate and extraction method of existing methods does not involve purification methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Imperata rhizome cut section is soaked in water for 2 hours, take 10kg and put it into the steam explosion reaction tank, feed steam under the condition of 9MPa, steam explosion treatment for 40 seconds, collect the steam explosion material and put it into the extraction tank, add 10 times the amount of water to reflux for extraction for 1 hour , plus 5 times the amount of water to extract once, add protease to the combined extract at 50°C to 10 activity units / L, enzymolysis for 4 hours, filter the enzymolysis solution with a 100,000 hollow cellulose membrane ultrafiltration, and collect the permeate Then use 10000 hollow cellulose membrane to concentrate by ultrafiltration, collect the concentrated solution and add twice the amount of 95% ethanol, fully precipitate, centrifuge, and vacuum-dry to obtain 540 g of polysaccharides from Imperata pratense, with a content of 81%.

Embodiment 2

[0016] Imperata rhizome cut section is soaked in water for 1 hour, take 10kg and put it into the steam explosion reaction tank, feed steam under the condition of 5MPa, steam explosion treatment for 60 seconds, collect the steam explosion material and put it into the extraction tank, add 6 times the amount of water to reflux for extraction for 1 hour , plus 6 times the amount of water to extract once, add protease to the combined extract at 45°C to 20 activity units / L, enzymolysis for 2 hours, filter the enzymolysis solution with a 60,000 hollow cellulose membrane ultrafiltration, and collect the permeate Concentrate again by ultrafiltration with a 5000 ℃ hollow cellulose membrane, collect the concentrated solution and add an equal volume of 95% ethanol, fully precipitate, centrifuge, and vacuum-dry to obtain 490 g of polysaccharides from Rhizoma Imperatae, with a content of 83%.

Embodiment 3

[0018] Imperata rhizome cut section is soaked in water for 1 hour, take 10kg and put it into the steam explosion reaction tank, pass steam under the condition of 10MPa, steam explosion treatment for 30 seconds, collect the steam explosion material and put it into the extraction tank, add 8 times the amount of water to reflux for extraction for 1 hour , plus 6 times the amount of water to extract once, add protease to the combined extract at 50°C to 15 activity units / L, enzymolysis for 3 hours, and filter the enzymolysis solution with a 60,000 hollow cellulose membrane for ultrafiltration, and collect the permeate Then use 10000 hollow cellulose membrane to concentrate by ultrafiltration, collect the concentrated solution and add 2 times the amount of 95% ethanol, fully precipitate, centrifuge, and vacuum dry to obtain 430 g of polysaccharides from Imperata pratense, with a content of 90%.

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Abstract

The invention discloses a method for extracting polysaccharide from imperata rhizome, which belongs to the biology technical field. The method comprises the following steps: segmentizing imperata rhizome and adding water for wetting, placing into a steam explosion reaction pot, introducing steam and carrying out a steam explosion treatment for 10-60 seconds under the pressure condition of 1-10MPa, collecting a steam exploded substrate and adding water for reflux extraction for 1-2 hours, adding protease in an extract for enzymatic hydrolysis, filtering an enzymatic hydrolysate and performing ultrafiltration by an ultrafilter membrane, collecting a permeate and concentrating by the ultrafilter membrane, collecting a concentrate and carrying out alcohol precipitation, vacuum drying to obtain imperata rhizome polysaccharide. The method of the invention has the advantages of simple operation, high recovery rate, low energy consumption, small pollution and easy industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting polysaccharides from the roots of Imperata coronata. Background technique [0002] Imperata cylindrica is the rhizome of the perennial grass Imperata cylindrica (scientific name: Imperata cylindrica). Traditional Chinese medicine believes that the rhizome of Imperatae is sweet and cold in nature and has the effects of cooling blood, stopping bleeding, clearing away heat and diuresis. Modern research has found that the polysaccharides of Imperatae Rhizome have the functions of improving human immunity and anti-tumor. [0003] Existing methods for extracting polysaccharides from Imperata coronata generally use water extraction, microwave or ultrasonic assisted extraction, and there are also enzymatic water extraction methods, followed by alcohol precipitation to remove protein. For example, Gou Jiangang et al. used the extraction temperature of 80°...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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