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Auxin transport protein gene of paddy rice and application thereof

An auxin, rice technology, applied in the field of genetic engineering, to achieve the effects of developed root system, increased lateral roots, and improved crop yield

Inactive Publication Date: 2012-03-14
HEBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there has been no report on the functional research of the auxin transport protein gene OsPIN3t in rice and its ability to improve rice drought tolerance

Method used

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  • Auxin transport protein gene of paddy rice and application thereof
  • Auxin transport protein gene of paddy rice and application thereof
  • Auxin transport protein gene of paddy rice and application thereof

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Experimental program
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Embodiment Construction

[0014] 1. Acquisition of genes

[0015] This auxin transport protein is a T-DNA inserted rice mutant obtained by the applicant during the construction and research of the T-DNA inserted rice mutant library. The flanking sequence of the T-DNA insertion site shows that the gene It encodes a presumed auxin transport protein in rice, the protein Accession No is gi|75109754, and its gene sequence number is AK063976. In order to obtain the gene, the applicant extracted the RNA of wild-type rice (Oryza sativa) Nipponbare, and used primers 5'-TCTAGAATGATATCCGGGCACGACT-3' and 5'-GAGCTCTCATAGTCCAAGAAGGATGTAGT-3' for RT-PCR amplification. The reaction system is as follows: Ultrapure Water 31ul, 10×GC Buffer II 5ul, dNTP Mixture 4ul, upstream and downstream primers 4ul, LA DNA Taq Polymerase 0.2ul, cDNA 2ul. The PCR program is: 5 minutes at 94°C, 30 seconds at 94°C, 50 seconds at 58°C, 120 seconds at 72°C, 25 cycles of the second to fourth steps, and 10 minutes at 72°C. The PCR product ...

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Abstract

The invention discloses an auxin transport protein gene of paddy rice and application thereof, and aims to provide an auxin transport protein gene of paddy rice, an encoding protein thereof and a method for improving the drought resistance of a plant by using the gene. The nucleotide sequence of the auxin transport protein gene of paddy rice provided by the invention is a DNA (Deoxyribose Nucleic Acid) in a sequence 1 in a sequence table; and an encoded amino acid sequence of the auxin transport protein gene is an amino acid residue sequence in a sequence 2 in the sequence table. As proved by experiments, the gene is overexpressed in paddy rice, so that the development of lateral roots and adventitious roots of paddy rice can be promoted, and the drought resistance of paddy rice can be improved under the action of drought stress. The auxin transport protein gene can be importantly applied to improving the drought resistance and abiotic stress resistance of paddy rice by adopting molecular breeding.

Description

technical field [0001] The invention relates to a gene encoding auxin transport protein in rice, which regulates growth and development and drought resistance of rice by regulating auxin transport in rice, and belongs to the technical field of genetic engineering. Background technique [0002] As an important plant hormone, auxin plays a very important role in regulating various growth and development processes and environmental responses of plants. The relative content of auxin in cells is directly related to the fate of plant cell growth and development, and polar transport is the main factor to maintain the concentration gradient of auxin. The transport of auxin in plants is an active transport process that requires energy. Its polar transport mechanism is relatively complicated, and its transport mode is different in different parts of the plant. The auxin is transported downward in the coleoptile and shoot tip, and in the The root is transported to the root tip, and th...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415A01H5/00
Inventor 朱正歌张文娇张倩闫书宁李晶晶孙宗修
Owner HEBEI NORMAL UNIV
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