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Application of cholesterol molecule imprinted membrane sensing electrode in detecting blood cholesterol

A technology of molecularly imprinted membranes and sensing electrodes, which is applied in the direction of measuring devices, analyzing materials, and material analysis through electromagnetic means, to achieve the effects of expanding the application range, preventing cracks, and increasing conductivity

Inactive Publication Date: 2014-02-19
无锡市金坤生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Due to the specificity and stability of molecularly imprinted polymers in the recognition of template molecules, their membranes are suitable as sensors with high sensitivity. However, there are many technical difficulties in the preparation of molecularly imprinted membranes. Find suitable functional monomers; due to the brittleness of the prepared membrane, a suitable cross-linking agent is required for curing; it is necessary to select a suitable eluent, not only to wash away the template molecules, but also to maintain the holes in the membrane shape, etc., its practical application is still very limited

Method used

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  • Application of cholesterol molecule imprinted membrane sensing electrode in detecting blood cholesterol
  • Application of cholesterol molecule imprinted membrane sensing electrode in detecting blood cholesterol
  • Application of cholesterol molecule imprinted membrane sensing electrode in detecting blood cholesterol

Examples

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Embodiment 1

[0033] (a) Electrode preparation.

[0034] Such as figure 1 with figure 2 As shown, the insulated electrode substrate 1 is taken, and the separated working electrode 4 and counter electrode 5 are coated with conductive silver paste by adopting screen printing technology. The semi-circular ring; corresponding to the working electrode 4 and the counter electrode 5 on the insulated electrode substrate 1, the working electrode connected 3 and the terminal 2 and the counter electrode connection 3 and the terminal 2 respectively printed in sequence; A layer of insulating layer 6 is coated on the surface of the working electrode connection wire 3 and the counter electrode connection wire 3 .

[0035] Wherein, the length of the electrode substrate 1 is 38mm, the width is 10mm, and the thickness is 0.5mm;

[0036] Terminal 2 has a length of 10mm and a width of 1mm;

[0037] The electrode connecting wire 3 has a length of 20 mm and a width of 1 mm;

[0038] The distance between th...

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Abstract

The invention provides application of a cholesterol molecule imprinted membrane sensing electrode in detecting cholesterol content in blood. Specific steps in the method are as follows: (1) treating a blood sample, that is, standing the blood sample at room temperature and carrying out centrifugation to extract serum if the blood sample is obtained by drawing blood from a person who is on an empty stomach, or carrying out anticoagulation by using heparin or EDTA (ethylene diamine tetraacetic acid) if the blood sample is blood plasma; (2) dropwise adding the blood sample obtained in step (1) onto the cholesterol molecule imprinted membrane sensing electrode, inserting the cholesterol molecule imprinted membrane sensing electrode into an amperometric sensor for determination, and recording readings. When used for detecting cholesterol content in blood, the molecule imprinted membrane sensing electrode has the advantages of low cost, high sensitivity, fast analysis speed, capacity of reducing sampling amount and avoiding tedious pretreatment of blood samples, etc.

Description

technical field [0001] The invention relates to measuring the content of cholesterol in blood, in particular to detecting the content of cholesterol in blood by using a cholesterol molecule imprinted film sensing electrode. Background technique [0002] Cholesterol plays an important physiological role in the human body. It plays an important role and function in forming bile acid, forming important components of cell membranes, and synthesizing hormones involved in the metabolism of various substances in the body. However, when it is excessive, it will lead to high cholesterol. Hyperemia has adverse effects on the body. Modern research has found that atherosclerosis, venous thrombosis, and hypercholesterolemia are closely related; high cholesterol can also have an adverse effect on the health of the body's bones, and a large intake of cholesterol can cause gaps between teeth and gums. The cells that will expand and prevent bacteria from entering the groove will lose their ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/416
Inventor 赵晓联周智慧朱秋劲王捷毛飞君
Owner 无锡市金坤生物工程有限公司
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