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Method for extracting tea saponin from Camellia oleiera Aberl. cake

An extraction method and tea saponin technology are applied in chemical instruments and methods, preparation of sugar derivatives, steroids, etc., to achieve the effects of convenient operation, simple process operation and cost reduction

Inactive Publication Date: 2012-02-15
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems existing in the extraction process of tea saponin in the dry cake of camellia oleifera, and provide a new method of mechanochemical extraction that is environmentally friendly, high in yield and simple in process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Put 10g of dry camellia oleifera cake and 0.4g of silica gel (300 mesh) into a ball mill, and ball mill at a speed of 2000rpm for 10min; take out the ball mill powder, add 52g of water to the obtained ball mill powder, stir for 10min, filter, take the filtrate, and put it on D-101 The macroporous resin column was eluted with 2 times column volume of 80wt% ethanol aqueous solution, and the eluate was collected, concentrated, evaporated to remove the solvent, and dried to obtain tea saponin with a detection yield of 8.56% by HPLC.

Embodiment 2

[0031] Put 10g dried camellia oleifera cake and 1.0g silica gel (300 mesh) into a ball mill, and ball mill at a speed of 2000rpm for 5 minutes; take out the ball mill powder, add 550g water to the obtained ball mill powder, and stir for 20 minutes; filter, take the filtrate, and put it on D-101 The macroporous resin column was eluted with 4 times column volume of 80wt% ethanol aqueous solution, and the eluate was collected, concentrated, evaporated to remove the solvent, and dried to obtain tea saponin with a detection yield of 10.64% by HPLC.

Embodiment 3

[0033] Put 10g dried camellia oleifera cake and 2g silica gel (300 mesh) into a ball mill, and ball mill at a speed of 1000rpm for 10min; take out the ball mill powder, add 120g water to the ball mill powder, and stir for 30min; filter, take the filtrate, and put it on AB-8 large The resin column was eluted with 4 times column volume of 80wt% ethanol aqueous solution, the eluate was collected, concentrated, evaporated to remove the solvent, and dried to obtain tea saponin with a detection yield of 16.05% by HPLC.

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Abstract

The invention discloses a method for extracting tea saponin from Camellia oleiera Aberl. cake. The extraction method comprises the following steps: putting Camellia oleiera Aberl. cake powder together with a solid reagent in a ball mill, carrying out ball milling, taking out ball milled powder, adding water to the ball milled powder, stirring, filtering, taking the filtrate to a macroporous resin column, eluting with an aqueous solution containing 80 wt% of ethanol, collecting an eluate, concentrating to evaporate a solvent, and drying to obtain the tea saponin. The method of the invention, which has the advantages of cheap raw material, high yield and high purity of the tea saponin, simple process, convenient operation, low cost, environment protection and the like, is a tea saponin preparation method with good popularization and application prospects.

Description

(1) Technical field [0001] The invention relates to a method for extracting tea saponin from dry cake of camellia oleifera. (2) Background technology [0002] Camellia oleiera (Camellia oleiera Aberl.) originates in my country. It mainly refers to the general term for plants with high seed oil content and economic cultivation value in the Camellia family Camellia. It is a unique "national-level characteristic resource" in China. Palm and coconut are known as the world's four largest woody oil plants. Camellia oleifera dry cake is the solid residue of camellia oleifera seeds after the oil is squeezed out, and tea saponin is its main chemical component. Theasaponin is a mixture of oleanane-type pentacyclic triterpenoid saponins with similar structures. Research in the past thirty years has shown that tea saponin is a pure natural non-ionic surfactant (Daily Chemical Industry, 2006, 36 (3), 174), and has antibacterial, anti-inflammatory, anti-viral, anti-oxidant, anti- High b...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07H1/08C07J63/00
Inventor 朱兴一苏为科王平谢捷
Owner ZHEJIANG UNIV OF TECH
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