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Modeling device for establishing bladder cancer orthotopic transplantation tumor animal model

An animal model and tumor transplant technology, which is applied in veterinary instruments, veterinary surgery, medical science, etc., can solve the problems of complicated operation, large damage to animal bladder mucosa, and long operation time, and achieves easy and convenient operation and shortens modeling. Operation time, the effect of injury stabilization

Inactive Publication Date: 2013-08-28
山西省肿瘤医院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention aims to solve the shortcomings of the existing bladder orthotopic transplantation animal model modeling methods, such as long operation time, complicated operation, and large damage to the animal bladder mucosa, and provides a method for establishing bladder cancer orthotopic transplantation animal models. modeling device

Method used

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  • Modeling device for establishing bladder cancer orthotopic transplantation tumor animal model
  • Modeling device for establishing bladder cancer orthotopic transplantation tumor animal model
  • Modeling device for establishing bladder cancer orthotopic transplantation tumor animal model

Examples

Experimental program
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Effect test

Embodiment 1

[0030] T739 mice, 70, female, weighing 20-24 grams, were randomly divided into an experimental group of 60 and a control group of 10, purchased from Beijing Huafukang Experimental Animal Technology Co., Ltd., production license number: SCXK (jing) 2009 -0004;

[0031] Mouse bladder cancer BTT cells: donated by Prof. Yang Xiaofeng, Department of Urology, Shanxi Medical University.

[0032] Mice were anesthetized by intraperitoneal injection of pentobarbital sodium at 60 mg / kg, placed in the supine position, and routinely disinfected the lower abdomen. The soft sleeve 5 of the sterile sleeve seat 4 was coated with liquid paraffin, and slowly inserted into the bladder cavity through the urethra. Draw out the urine. Slowly insert the needle core 1 with a deflection angle of 5-7° into the soft cannula 5 of the cannula seat 4, fix the cannula seat 4 with the left hand, and make the head of the soft cannula 5 rest on the bladder wall of the animal. Turn the needle core 1 clockwise,...

Embodiment 2

[0036] 70 Bslb / c-nu-nu mice, female, weighing 20-22 grams, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., license number: SCXK (jing) 2006-0009;

[0037] Cell line: human bladder cancer T24 cell line, purchased from Shanghai Biotechnology Co., Ltd., Chinese Academy of Sciences.

[0038] Mice were anesthetized by intraperitoneal injection of pentobarbital sodium at 60 mg / kg, placed in the supine position, and routinely disinfected the lower abdomen. The soft sleeve 5 of the sterile sleeve seat 4 was coated with liquid paraffin, and slowly inserted into the bladder cavity through the urethra. Draw out the urine. Slowly insert the needle core 1 with a deflection angle of 5-7° into the soft cannula 5 of the cannula seat 4, fix the cannula seat 4 with the left hand, and make the head of the soft cannula 5 rest on the bladder wall of the animal. Turn the needle core 1 clockwise, pull out the needle core 1 after 3-5 turns (1080~1800°), and immedi...

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Abstract

The invention discloses a modeling device for establishing a bladder cancer orthotopic transplantation tumor animal model, solving the defects of long surgical time, complex operation, large animal bladder mucosa hurt and the like of the traditional modeling method. The modeling device comprises a needle seat fixedly arranged at the front end and a sleeve base provided with a cavity, wherein the front end of the sleeve base is fixedly provided with a soft sleeve communicated with the cavity, the front end of the needle base is sleeved and inserted in the cavity of the sleeve base and positioned by using a positioning baffle ring arranged on the needle base, a needle core on the needle base is sleeved and inserted in the soft sleeve of the sleeve base, and the length of the needle point ofthe needle core, exposed out of the end part of the soft sleeve, is 0.5-1.0mm, a break angle of 5-7 DEG is arranged at a place of the needle core, which is at a 1.5-2.0cm distance of the needle point, and a marking groove is arranged on the positioning baffle ring. The modeling device has the advantages of skilled design, simple structure and easiness in operation, greatly shortens the surgical time, and has little damage to the animal bladder mucosa and a tumor formation rate of reaching 100 percent.

Description

technical field [0001] The invention relates to a medical device, in particular to a modeling device for establishing an animal model of orthotopic transplanted tumor of bladder cancer. Background technique [0002] Bladder cancer is the most common tumor of the urinary system, and more than 90% of patients are transitional cell carcinoma, of which superficial bladder cancer accounts for 70-85%. Due to the multifocal nature of bladder cancer lesions and the implantation of tumor cells in the wound after surgery, the postoperative tumor recurrence rate is as high as 50-70%. Nowadays, people establish bladder cancer animal models to study the etiology, pathology and other biological behaviors of bladder cancer, and the efficacy of intravesical infusion chemotherapy, immunotherapy and biological therapy, which provides a scientific basis for clinical treatment and prevention of postoperative recurrence. To carry out research on the prevention and treatment of bladder cancer, i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61D1/00
Inventor 任连生杨喜花张蕻赵莉莉王靖
Owner 山西省肿瘤医院
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