Method for preparing CysC-paired monoclonal antibody
A monoclonal antibody and cystatin technology, applied in the field of protein engineering, can solve the problems of difficult to obtain paired monoclonal antibodies, heavy workload, narrow screening range, etc., and achieve the effect of increasing the probability, reducing the workload, and expanding the screening range
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[0023] 1. Experimental materials
[0024] 1) Expression vector: pET28a(+) was purchased from (Novagen); Figure 4 As shown, also refer to the product manual provided by Novagen.
[0025] 2) Expression host bacteria: Escherichia coli BL21(DE3), purchased from Beijing Tiangen Biotechnology Co., Ltd.;
[0026] 3) Yeast powder and peptone: both are products of Oxoid Company;
[0027] 4) The CysC gene was synthesized by Shanghai Xuguan Biotechnology Co., Ltd.; the CysC gene sequence with NcoI and XhoI restriction sites is shown in SEQ ID NO: 1 ( Figure 5 ), italics are the added NcoI and XhoI restriction sites.
[0028] 5) SP 2 / 0 myeloma cells were purchased from the Cell Center of Shanghai Institute of Biochemistry and preserved by the R&D Department of our company;
[0029] 6) Balb / c mice were purchased from the Experimental Animal Center of Chinese Academy of Medical Sciences.
[0030] 7) Fetal bovine serum: purchased from Hangzhou Sijiqing Company;
[0031] 8) DMEM mediu...
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