Closely interlocked molecular mark of rice new gene source-sink 1 (SS1)

Abstract

The invention relates to a molecular marking method of a rice new gene source-sink 1 (SS1), and belongs to the fields of super-high-yield breeding of rice and molecular genetics. The essence of the invention is as follows: according to the interlocking separation law, an F2 secondary separation group constructed by autocopuation of a near-isogenic line carrying a SS1 heterozygous segment is takenas a test material for constructing a set of crossing and stacking system of a target segment, phenotype is further combined for performing high-precision interlocking analysis on a target gene, through two years of two-point repeated identification, the SS1 is finally precisely positioned in an interval of 55Kb on the fourth chromosome and a PCR (polymerase chain reaction)-based molecular mark FL41 which is closely interlocked with the SS1 is further obtained. Through the application of the molecular marking method in the super-high-yield breeding of rice, genotype individuals which can simultaneously affect the characters of a library (the number of grains per panicle) and a source (the flag leaf width and the leaf area) can be fast accurately identified, the early generation selection of breeding material can be performed further and the process of the super-high-yield molecular breeding of rice can be greatly accelerated.

Description

technical field [0001] The invention relates to a tightly linked molecular marker for controlling rice sink-source new gene SS1, which belongs to the field of rice super-high-yield breeding and molecular genetics, and is suitable for introducing a new yield-related gene SS1 in rice high-yield breeding, and using tightly linked molecular markers The gene was used for assisted selection breeding for rice sink-source related traits. Background technique [0002] The level of rice yield mainly depends on the strength of source, sink and flow and the degree of coordination between them. Among them, the panicle traits, especially the total number of grains per panicle, are the main sink traits of photosynthetic product accumulation, and the inverted three leaves are especially Flag leaf size is a major source trait for photosynthetic product production, and the number of large vascular bundles in panicle stems is often considered a "flow" trait for transporting assimilates from le...

AI Technical Summary

Problems solved by technology

Although conventional phenotypic identification can select source traits such as leaf width and leaf size, it is impossible to differentiate and utilize different types of sink-source-related gene loci; only molecular markers can be used to simultaneously control sink traits. Only by targeted selection of gene loci for source and source traits can the efficient breeding of super-high-yield varieties with coordinated sink-source be realized

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