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Method for establishing zebra fish P-glycoprotein inhibitor screening model and application thereof

A technique for glycoprotein inhibitors and method establishment, which is applied in the field of drug screening to achieve the effects of strong comparability, simple experimental analysis methods, and less dosage

Active Publication Date: 2014-04-16
上海环特智鱼优检生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, there are no reports on the screening of Pgp inhibitors using the whole zebrafish model at home and abroad, and a few studies only explore the toxicity mechanism of compounds (poisons) to zebrafish Pgp or conduct genetic analysis on MDR genes [17-19]

Method used

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  • Method for establishing zebra fish P-glycoprotein inhibitor screening model and application thereof
  • Method for establishing zebra fish P-glycoprotein inhibitor screening model and application thereof
  • Method for establishing zebra fish P-glycoprotein inhibitor screening model and application thereof

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Embodiment Construction

[0055] Reagents and instruments

[0056] Rhodamine B was purchased from Shanghai Jingchun Industrial Co., Ltd. Other reagents were provided by Beijing Dingguo Changsheng Technology Co., Ltd.

[0057] Dissecting microscope (SMZ645, Nikon, Japan); motorized focusing zoom fluorescence microscope (AZ100, Nikon, Japan); multi-functional microplate analyzer (Mithras LB940, Berthold Technologies, Germany).

[0058] The purpose of the present invention is to provide a method for establishing a screening model for P-glycoprotein inhibitors in living zebrafish, and at the same time provide a method for screening and evaluating P-glycoprotein inhibitors using the model. The method provided by the invention has the advantages of rapidity, reliability, economy, high efficiency, high throughput and the like.

[0059] One, the present invention provides a kind of establishment method of live zebrafish P-glycoprotein inhibitor screening model, concrete steps are:

[0060] 1 zebrafish selec...

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Abstract

The invention relates to a method for establishing a zebra fish P-glycoprotein inhibitor screening model and evaluating or screening a P-glycoprotein inhibitor. The method mainly comprises the steps of selecting the zebra fish, treating compounds, analyzing images and / or analyzing microporous plates. The method has the advantages of economy, simpleness and convenience, high speed, high efficiency, small using amount of the compounds and the like. By the method, the speed of evaluating or screening the P-glycoprotein inhibitor can be increased obviously; and important significance for accelerating screening and evaluation of the P-glycoprotein inhibitors on a large scale is achieved.

Description

technical field [0001] The invention belongs to the field of drug screening (evaluation), and in particular relates to a method for establishing a simple, economical and high-throughput zebrafish P-glycoprotein inhibitor screening model and a method for screening and evaluating P-glycoprotein inhibitors using the animal model method. Background technique [0002] Malignant tumors are common diseases that seriously endanger human health. According to the report of the World Health Organization (WHO), at present, about 10 million cancer patients are newly discovered every year in the world, and the number of people who die from cancer is 6 to 7 million. In China, the cancer mortality rate has increased by nearly 30% in the past 20 years. [1] . [0003] Chemotherapy is an important means of treating cancer, but a common and difficult problem in chemotherapy is the generation of tumor multidrug resistance (MDR), that is, tumor cells are resistant to multiple chemical agents un...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N21/64
Inventor 朱晓宇李春启郭胜亚
Owner 上海环特智鱼优检生物科技有限公司
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