A kind of purification method of Grifeng element
A purification method and pressure technology, which is applied in the field of medicine, can solve the problems of not obtaining high-purity monomer compounds, using toxic and harmful reagents, and being unsuitable for industrial production, etc., and achieve the effect of short preparation cycle, short extraction time and large preparation volume
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Embodiment 1
[0016] Take the single-leaf Grifeng root, crush it to 20 mesh, take 1kg and put it into the extraction kettle, set the equipment parameters as follows: extraction temperature 50°C, extraction pressure 30MPa, desorption kettle Ⅰ temperature 33℃, pressure 19MPa, desorption kettle Ⅱ temperature The temperature is 27°C, the pressure is 5MPa, and the supercritical CO 2 , to regulate CO 2 The flow rate was 18 L / h, entraining 440 ml of 70% propylene glycol, extracting for 60 min, analyzing and recovering the reagent, and obtaining the extract. Dissolve and disperse the extract in water, pass it through an AB-8 macroporous adsorption resin column, elute with water until colorless, then elute with 60% ethanol, collect the eluate, concentrate under reduced pressure to obtain the extract. Mix chloroform, methanol, and water in a volume ratio of 6:4:4, take the upper phase as the stationary phase, and the lower phase as the mobile phase, turn on the host of the high-speed countercurrent ...
Embodiment 2
[0018] Take the single-leaf Gelifeng root, crush it to 20 mesh, take 1kg and put it into the extraction kettle, set the equipment parameters as follows: extraction temperature 55°C, extraction pressure 25MPa, analysis kettle Ⅰ temperature 35℃, pressure 23MPa, analysis kettle Ⅱ temperature 25°C, pressure 4MPa, supercritical CO 2 , to regulate CO 2 The flow rate was 18L / h, 520ml of 70% propylene glycol was entrained, extracted for 120min, and the reagent was analyzed and recovered to obtain the extract. The extract was dissolved and dispersed in water, passed through a DM310 macroporous adsorption resin column, first eluted with water until it was colorless, and then eluted with 55% ethanol, the eluate was collected, concentrated under reduced pressure to obtain the extract. Mix chloroform, methanol, and water in a volume ratio of 5:3:3, take the upper phase as the stationary phase, and the lower phase as the mobile phase, turn on the host of the high-speed countercurrent chrom...
Embodiment 3
[0020] Take the single-leaf Grifeng root, crush it to 20 mesh, take 1kg and put it into the extraction kettle, set the equipment parameters as follows: extraction temperature 45°C, extraction pressure 32MPa, analysis kettle Ⅰ temperature 38℃, pressure 21MPa, analysis kettle Ⅱ temperature 30°C, pressure 6MPa, supercritical CO 2 , to regulate CO 2 The flow rate was 18 L / h, entraining 600 ml of 70% propylene glycol, extracting for 30 min, analyzing and recovering the reagent, and obtaining the extract. Dissolve and disperse the extract in water, pass it through a HZ830 macroporous adsorption resin column, elute with water until it becomes colorless, then elute with 50% ethanol, collect the eluate, and concentrate under reduced pressure to obtain the extract. Mix chloroform, methanol, and water in a volume ratio of 4:3:3, take the upper phase as the stationary phase, and the lower phase as the mobile phase, turn on the host of the high-speed countercurrent chromatograph, adjust t...
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