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Method for culturing red-petiole taros

A technology of red stems and culture medium, applied in the field of tissue culture of herbaceous plants, to achieve the effects of increasing the speed of reproduction, increasing the yield, and increasing the survival rate

Active Publication Date: 2013-03-06
上海上房园艺有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tissue culture report of Colocasia 'Red Stem' in China has not been seen yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for cultivating taro, the method comprises the following steps:

[0027] (1) Aseptic treatment

[0028] In spring, the small buds of the basal taro were excavated when they first germinated, rinsed with tap water for 2 hours, then placed on a super-clean workbench, soaked in 75wt% ethanol for 30s, soaked in 1wt‰ of mercury chloride solution for 15min, and rinsed with sterile water After 5-6 times, dry the surface water with sterile filter paper, cut the base of the buds into 1cm lengths and inoculate them on the bud induction medium MS+6-BA5.0mg / L+NAA0.5mg / L;

[0029] (2) Differentiation and proliferation of buds

[0030] 3 weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after 2 weeks. After culturing for 1 month, the callus with buds was cut and placed Proliferation medium MS+6-BA2.0mg / L+NAA0.2mg / L was cultured, and th...

Embodiment 2

[0038] A method for cultivating taro, the method comprises the following steps:

[0039] (1) Aseptic treatment

[0040] In spring, the small buds of the basal taro were excavated when they first germinated, rinsed with tap water for 2 hours, and then placed on a super-clean workbench, soaked in 75wt% ethanol for 20s, 1wt‰ of mercuric chloride solution for 10min, and rinsed with sterile water After 5-6 times, dry the surface water with sterile filter paper, cut the base of the buds into 1cm lengths and inoculate them on the bud induction medium MS+6-BA1.0mg / L+NAA0.1mg / L;

[0041] (2) Differentiation and proliferation of buds

[0042] 3 weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after 2 weeks. After culturing for 1 month, the callus with buds was cut and placed Proliferation medium MS+6-BA2.0mg / L+NAA0.2mg / L was cultured, and there w...

Embodiment 3

[0050] A method for cultivating taro, the method comprises the following steps:

[0051] (1) Aseptic treatment

[0052] Dig out the small buds at the base of the red taro in spring when it just germinates, wash it with tap water for 2 hours, put it on the ultra-clean workbench, soak it in 75wt% ethanol for 40s, soak it in 1wt‰ mercury chloride solution for 20 minutes, and wash it with sterile water After 5-6 times, dry the surface water with sterile filter paper, cut the base of the buds into 1cm lengths and inoculate them on the bud induction medium MS+6-BA3.0mg / L+NAA0.3mg / L;

[0053] (2) Differentiation and proliferation of buds

[0054]3 weeks after the budlets were inoculated on the budlet induction medium, the base of the budlets began to expand, and yellow-green protrusions appeared, and obvious callus tissue was visible after 2 weeks. After culturing for 1 month, the callus with buds was cut and placed Proliferation medium MS+6-BA3.0mg / L+NAA0.3mg / L was cultured, and t...

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PUM

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Abstract

The invention relates to a method for culturing red-petiole taros. The method comprises the following steps: (1) obtaining sterile material; (2) differentiating and proliferating buds; (3) culturing adventitious bud strong seedlings; (4) striking roots and culturing; and (5) acclimatizing and transplanting. Compared with the prior art, the method has the advantages that the propagation speed and the neatness of seedlings can be greatly increased through a tissue culture technology, the trait of a female patient is better retained, and the survival rate can be increased, therefore, the yield is increased, and the transplanting survival rate can be up to 95%.

Description

technical field [0001] The invention relates to a method for tissue culture of herbaceous plants, in particular to a method for cultivating taro. Background technique [0002] Colocasia 'Red Stem' is a perennial herb with a plant height of 50-60 cm. The petiole is purple, and it is an excellent variety artificially bred. Red stalk taro likes high temperature and humidity, and has strong adaptability. It can be planted on the shore or in wetlands, and has a good ornamental effect. As the species introduced from abroad, the number of introductions is small, the ramets are slow, the market demand is large, and the supply of seedlings is limited. Through tissue culture technology, the propagation speed and the uniformity of seedlings are greatly improved, and the original female parent traits are better maintained. There is no report on the tissue culture of Colocasia 'Red Stem' in China. Contents of the invention [0003] The purpose of the present invention is to provide...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 陈建华黄建荣沈勤
Owner 上海上房园艺有限公司
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