An automatic analyzer and analysis method for reducing sugar and water-soluble total sugar content

An automatic analyzer, technology of total sugar content, applied in the measurement of color/spectral characteristics, etc., can solve the problems of operator damage, cumbersome steps, high price, etc., and achieve the effect of high degree of automation, saving chemical reagents, and simple instrument

Inactive Publication Date: 2014-10-22
BEIJING JITIAN INSTR CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the determination of reducing sugar and water-soluble total sugar content in food, plants and other substances mostly relies on manual operation, the steps are cumbersome, human error is large, precision is poor, test speed is slow, and toxic reagents may cause damage to operators
At present, high performance liquid chromatography can be used to measure reducing sugar, but it cannot directly measure the content of water-soluble total sugar, and the instrument is expensive and the test speed is slow
At present, there is a commercial bubble gap continuous flow analyzer on the market, which can directly measure the content of reducing sugar and water-soluble total sugar, but it needs to have an additional bubble adding device. It is easy to enter a stable state, and it is difficult for analysts to master its operation

Method used

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  • An automatic analyzer and analysis method for reducing sugar and water-soluble total sugar content
  • An automatic analyzer and analysis method for reducing sugar and water-soluble total sugar content
  • An automatic analyzer and analysis method for reducing sugar and water-soluble total sugar content

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Experimental program
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Effect test

Embodiment 1

[0033] see figure 1 , the automatic analyzer of measuring reducing sugar and water-soluble total sugar content of the present invention comprises sampling device and color detection device; There is a first pump tube 12, and the sampling needle 2 of the autosampler 1 is connected to the F port of the multi-way valve 20 through the first pump tube 12, and the A port of the multi-way valve 20 is connected to the waste liquid tank 36. A first back pressure regulator 21 is arranged between them; a color detection device includes a second peristaltic pump 4, and the second peristaltic pump 4 is provided with a second pump tube 13, a third pump tube 14, a fourth pump tube 15, a fifth pump tube The pump tube 16, the sixth pump tube 17, the seventh pump tube 18, and the two ends of the sampling loop 19 are connected to the B port and the E port of the multi-way valve 20, and the D port of the multi-way valve 20 is connected to the carrier solution bottle. 5 is connected through the s...

Embodiment 2

[0039] Use the automatic analyzer of reducing sugar and water-soluble total sugar, the first to the seventh pump tube internal diameter is 0.38mm, the first peristaltic pump, the second peristaltic pump pump speed is 15 rev / min, and the capillary length of described sampling ring is 1m, the capillary length of the first braided reactor is 0.5m, the capillary length of the second braided reactor is 1m, the capillary length of the online heating digestion module is 5m, the capillary length of the online heating color development module is 4m, and the inner diameter of the connecting capillary is 0.5mm . The method for measuring reducing sugar comprises the steps in turn:

[0040] (1) The liquid to be tested and the deionized water in the second container bottle 6 are mixed through the first tee, and the mixed liquid is injected into the on-line heating and digestion module 23, and the temperature of the on-line heating and digestion module is 80°C;

[0041] (2) the liquid obtai...

Embodiment 3

[0051] The automatic analyzer of reducing sugar and water-soluble total sugar, the inner diameter of the first to seventh pump tubes is 1.85mm, the pump speed of the first peristaltic pump and the second peristaltic pump is 60 rpm, the sampling loop The length of the capillary is 4m, the length of the capillary of the first braided reactor is 3m, the length of the capillary of the second braided reactor is 4m, the length of the capillary of the online heating and digestion module is 10m, the length of the capillary of the online heating and color development module is 9m, and the inner diameter of the connecting capillary is 1.0mm. The method for measuring reducing sugar comprises the steps in turn:

[0052] (1) The liquid to be tested and the deionized water in the container bottle (6) are mixed through the first tee, and the mixed solution is injected into the on-line heating and digestion module (23), and the temperature of the on-line heating and digestion module is 120°C;...

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Abstract

The invention relates to an automatic analyzer and a determination method for determining the content of reducing sugars and water-soluble total sugars, including a sampling device and a color detection device; the sampling device includes an automatic sampler, a first peristaltic pump, and the first The peristaltic pump is provided with a first pump tube, the injection needle of the automatic sampler is connected to the F port of the multi-way valve through the first pump tube, and the A port of the multi-way valve is connected to the waste liquid tank, and the There is a first back pressure regulator. The color detection device includes a second peristaltic pump, and the second peristaltic pump is provided with a second pump tube, a third pump tube, a fourth pump tube, a fifth pump tube, a sixth pump tube, and a seventh pump tube , the two ends of the sampling loop are connected to the B port and the E port of the multi-way valve, the D port of the multi-way valve is connected to the carrier solution through the second pump tube, and the multi-way valve’s Port C is sequentially connected with the first three-way, the online heating and digestion module, the second three-way, the first weaving reactor, the third three-way, the fourth three-way, the online heating and color development module, the fifth three-way, the second Braided reactors, flow cells, detectors, data link cables, and computer processing systems. The invention can continuously and automatically test samples, and the test is accurate and fast.

Description

technical field [0001] The invention relates to a fully automatic flow injection analyzer, in particular to an automatic analyzer for reducing sugar and water-soluble total sugar content and an analysis method thereof. Background technique [0002] At present, the determination of reducing sugar and water-soluble total sugar content in food, plants and other substances mostly relies on manual operation, the steps are cumbersome, human error is large, precision is poor, test speed is slow, and toxic reagents may cause damage to operators . At present, reducing sugar can be determined by high performance liquid chromatography, but the content of total water-soluble sugar cannot be directly determined, and the instrument is expensive and the test speed is slow. At present, there is a commercial bubble gap continuous flow analyzer on the market, which can directly measure the content of reducing sugar and water-soluble total sugar, but it needs to have an additional bubble addi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 王丽平肖靖泽赵萍魏月仙顾爱平
Owner BEIJING JITIAN INSTR CO LTD
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