Method for detecting residues of five kinds of sulfa veterinary medicines in animal-based functional food

Abstract

The invention discloses a method for detecting residues of five kinds of sulfa veterinary medicines in an animal-based functional food. The method comprises the following steps of: (1) extracting a sample: weighing a proper amount of sample, extracting for 2 to 4 times by using acetonitrile as a solvent in a vortex oscillation way, combining acetonitrile solution and placing in a liquid separation funnel for later use; (2) purifying the sample: extracting the acetonitrile solution obtained in the step (1) by using normal hexane, concentrating the acetonitrile solution on the bottom layer and dissolving by using 10 percent acetonitrile water, and filtering; and (3) testing the residual amount of five kinds of sulfa veterinary medicines in the sample by using a high performance liquid chromatography-mass spectrometry-mass spectrometry (HPLC-MS-MS) system. By the test method, the separability of the five kinds of the sulfa veterinary medicine residues in the functional food is high, the sensitivity is high, and the selectivity and the specificity are high; matrix interference and false positive can be eliminated effectively, and the minimum detection concentration is low; furthermore, in a sample treatment process, the degressive amount of acetonitrile is used for extracting, so that the recovery rate of targets is guaranteed, the using amount of organic solvents is reduced, the emission of waste liquid is reduced and the detection cost is saved.

Description

technical field [0001] The invention relates to a method for detecting veterinary drug residues in animal-derived functional foods, in particular to a method for detecting sulfonamide veterinary drug residues. Background technique [0002] Over the past 30 years of reform and opening up, the functional food industry, as a sunrise industry in the 21st century, has shown more and more vitality. Functional foods have also gradually entered the lives of the broad masses of the people. It played an important role. [0003] The production and preparation of animal-derived functional foods such as calcium powder, fish oil and honey are mostly based on livestock, aquatic animals and semi-finished products, while sulfa drugs are widely used in the prevention and treatment of diseases due to their low cost and good disease resistance. Treatment of bacterial infectious diseases of livestock and aquatic products, which may produce a variety of sulfonamide residues. The direct toxic ef...

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Problems solved by technology

[0005] In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a method for effectively detecting sulfadiazine (SDZ), sulfamethazine (SMR), sulfamethazine (SDM), sulfaquinoxaline (SQX) and sulfadimethoxine (SDT) five kinds of sulfonamide veterinary drug residue method to solve the problem that the minimum detection concentration in the prior art is high, matrix interference cannot be effectively eliminated, false positives are prone to occur and the amount of organic solvent used is large and other shortcomings

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