Mice model of orthotopic left lung transplantation and construction method thereof

Abstract

The invention belongs to the technical field of medical animal models and in particular relates to a mice model of orthotopic left lung transplantation and a construction method thereof. As a research tool, the model duplicates the processes of such diseases as acute lung injury, chronic immune injury, pulmonary fibrosis and the like, is mainly used in the field of lung transplantation and can be used in certain fields in the subjects such as immunology, pathophysiology and the like. The construction method of the model comprises the following steps: preparing cuffs; building a donor lung perfusion system; narcotizing a mice and carrying out endotracheal intubation and mechanical ventilation; performing an operation on a donor to obtain a donor lung; putting the cuffs in the donor lung; and performing an operation on a receptor and carrying out orthotopic transplantation on the left lung in the chest of the receptor. Important improvements are performed on the aspects of building the donor lung perfusion system, carrying out endotracheal intubation on the mice, perfusing the donor lung, putting the cuffs, tallying a pulmonary vessel with a bronchus by virtue of a cuff technique. Practices show that compared with the existing methods, the construction method is easier to master and operate, is subjected to less influence of the personal factors and is closer to the ideal condition that animal models are simple to operate and have good repeatability.

Description

technical field [0001] The invention belongs to the technical field of medical animal models, and in particular relates to a mouse orthotopic left lung transplantation model and a construction method thereof. Background technique [0002] In animal experiments, mice have many advantages over rats. Not only are there abundant reagent resources, many strains to choose from, and low experimental consumption, but they also have better gene control and can easily obtain a variety of transgenic and gene knockout strains. Compared with other mouse lung disease models, such as ventilator lung injury and lipopolysaccharide inhalation injury, the process of acute lung injury, chronic immune injury, and pulmonary fibrosis replicated in this model has the advantages of a clear starting point and easier control of background noise. These advantages can meet the special requirements of some experimental designs, making it have better application prospects in the field of lung transplantat...

AI Technical Summary

Problems solved by technology

Although researchers in the United States and Switzerland have proposed their own solutions, none of them meet the modeling requirements of "simple and reproducible"