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Nile tilapia cholecystokinin and coding nucleic acid thereof and application of functional octopeptide

A technology of Nile tilapia and cholecystokinin, which is applied in the field of new protein and its encoding nucleic acid of Nile tilapia, can solve the problems of undiscovered tilapia feeding and gastrointestinal digestion of short peptides

Inactive Publication Date: 2011-11-16
SUN YAT SEN UNIV
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  • Abstract
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Problems solved by technology

[0004] In the existing research, no short peptides that can regulate tilapia feeding and gastrointestinal digestion have been found

Method used

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  • Nile tilapia cholecystokinin and coding nucleic acid thereof and application of functional octopeptide
  • Nile tilapia cholecystokinin and coding nucleic acid thereof and application of functional octopeptide
  • Nile tilapia cholecystokinin and coding nucleic acid thereof and application of functional octopeptide

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Embodiment Construction

[0029] The present invention uses RT-PCR and RACE techniques for cloning. First, use Invitrogen’s Trizol Reagent to extract total brain RNA, then use Toyabo’s Rever Tra Ace-a-Tm First Strand cDNA Synthesis Kit for RT-PCR, and finally use the designed degenerate primers to carry out the cDNA part of the gene Fragment cloning. After obtaining partial fragments, use the TdT tailing kit of TaKaRa Company to add a piece of dCTP to the 3' end of cDNA, design cDNA 5' end-specific primers, and perform 5' RACE together with adapter primers AAU and AUAU. The product SMART 3' RACE reverse transcriptase SMARTScribe Reverse Transcriptase from CLONTECH was used for reverse transcription, and the linker primer UPM (including Long-UPM and Short-UPM) was used for 3'RACE. Finally, specific primers were designed in the 3' and 5' non-coding regions to clone the open reading frame ORF and determine the sequence.

[0030] Below in conjunction with embodiment, further illustrate the present invent...

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Abstract

The invention provides Nile tilapia cholecystokinin and a nucleic acid sequence, a cloning method and application thereof. In the invention, the amino acid sequence for coding the Nile tilapia cholecystokinin is shown as SEQ ID NO:1. The invention also provides application of a CCK(Cholecystokinin)-8 octopeptide sequence in digestion ingestion, and the fact that the CCK-8 polypeptide can promote pyloric caecum to secrete amylase, trypsin and pepsinogen and promote hypothalamus to secrete NPY (neuropeptide Y) and orexin is discovered after being abdominally injected into tilapia. The invention provides a theoretical basis for clarifying an effect of CCK in a fish digestion and ingestion process and provides some gene resources for developing novel fish feed additives in the future.

Description

technical field [0001] The invention relates to a new protein of Nile tilapia and its encoding nucleic acid, and at the same time, the invention also relates to the application of an octapeptide residue of the new protein. Background technique [0002] Tilapia ( Tilapia ), commonly known as African crucian carp, belongs to Perciformes, Perciformes, Cichlidae, and Tilapia (also known as Cichlidae, Cichlids). As a substitute for cod, tilapia is increasingly in demand in the international market. In the global freshwater fish trade, tilapia occupies the third place, second only to salmon and trout, and has extremely high economic value. With the continuous expansion of breeding and export scale, the requirements for bait configuration are also increasing. Due to the continuous increase in feed costs, research tends to use plant protein instead of fish meal to reduce costs. However, when fish eat plant protein feed, they will reduce food intake and lose weight, which is contr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/595C12N15/16C12N15/10A23K1/16A23K20/00A23K50/80
Inventor 李文笙
Owner SUN YAT SEN UNIV
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