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Plant low potassium sensitive correlated protein AtLKR1, its coding gene and application

A technology encoding genes and proteins, applied in the direction of plant gene improvement, application, plant peptides, etc., to achieve the effect of improving tolerance

Active Publication Date: 2011-11-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although increasing the amount of potassium fertilizer application can greatly increase crop yields, there are certain limitations to importing a large amount of potassium fertilizer both economically and in the long-term strategic sense.

Method used

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  • Plant low potassium sensitive correlated protein AtLKR1, its coding gene and application
  • Plant low potassium sensitive correlated protein AtLKR1, its coding gene and application
  • Plant low potassium sensitive correlated protein AtLKR1, its coding gene and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1, the acquisition of proteins related to plant potassium nutrition and their coding genes

[0044] Total RNA was extracted from 7-day-old Arabidopsis (Columbia type, also known as Col-0 ecotype) seedlings (purchased from the Arabidopsis Biological Resource Center, ABRC), and the RNA was digested with DNase from Takara Company. The digested DNA was reverse-transcribed to obtain cDNA. Using the cDNA as a template, a pair of primers respectively equipped with Xba I / EcoR I and Sac I / Sal I restriction sites were used for PCR amplification to obtain the full-length CDS of genes related to potassium nutrition. The primer sequences are as follows:

[0045] Primer1: 5′-AAtctagagaattcATGAGTGGAAGCAGAAGGAAGGC-3′(Xba I / EcoR I)

[0046] Primer2: 5′-GAgagctcgtcgacTTATTGCTTTTGTTTCTTCAGCGG-3′(Sac I / Sal I)

[0047] The high-fidelity Pyrobest DNA polymerase from Takara Company was used for amplification. The amplification system was 50 μL, containing 5.0 μL of 10×Pyrobest PCR ...

Embodiment 2

[0049] Example 2. Functional verification of proteins related to plant potassium nutrition and their coding genes

[0050] 1. Identification of Mutants

[0051] T-DNA insertion mutants numbered SALK 058629 (lkr1-a) and SALK_014699 (lkr1-b) were purchased from ABRC (Arabidopsis Biological Resource Center). The identification primers of their respective DNA levels are

[0052] SALK_058629LP: 5-TGCCAAGTGAAAAATATAATGGCA-3,

[0053] RP: 5-CGTCACAAAATGGTCGAACAGG-3

[0054] SALK_014699LP: 5-TTCAAACCGAGTTTGAGGATG-3

[0055] RP: 5-TATCCATGAACGCTTTCGAAC-3

[0056] In order to further determine whether it is knocked out at the transcriptional level, we grown Arabidopsis wild-type (Columbia type) and mutant lkr1 materials (lkr1-a and lkr1-b) on MS medium for one week, and sampled (about 100mg) Extract RNA.

[0057] The composition of normal MS medium is: 1650mg NH per liter solution 4 NO 3 , 1900mg KNO 3 , 370mgMgSO 4 ·7H 2 O, 170mg KH 2 PO 4 , 440mg CaC...

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Abstract

The invention discloses a plant low potassium sensitive correlated protein AtLKR1, its coding gene and application. The protein is the protein of the following 1) or 2): 1) a protein composed of an amino acid sequence as shown in Sequence 2 of a sequence table; 2) a plant low potassium sensitive correlated protein derived from 1) through the replacement and / or deletion and / or addition of one or more amino acid residues in the amino acid residual sequence as shown in Sequence 2 of the sequence table. After the gene AtLKR1 is expressed in a target plant, the plant shows the low potassium sensitivity, and can be treated as an indication plant for soil potassium index for an early warning of low potassium in soil; otherwise, knocking out the AtLKR1 gene provided by the invention or reducing the AtLKR1 gene expression level, the plant shows low potassium resistant property. The gene provided by the invention is of great significance for the cultivation of new low potassium tolerant species and the research of plants' low potassium tolerance.

Description

technical field [0001] The present invention relates to plant low potassium sensitive related protein AtLKR1 and its coding gene and application. Background technique [0002] Potassium is one of the most abundant cations in plant cells and one of the three major nutritional elements necessary for plant growth, playing an important role in the entire life of plants. It not only participates in many physiological and biochemical metabolic processes of plants, but also has an important impact on plant growth and development, yield formation, etc. by regulating photosynthesis, respiration and the functions of many enzyme systems. [0003] One of the important factors restricting the development of crop production in my country is that most of the cultivated land in our country lacks potassium and the resources of potassium fertilizer are extremely scarce. Although increasing the amount of potassium fertilizer application can greatly increase crop yields, importing a large amou...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21A01H5/00
Inventor 武维华刘丽丽任慧敏
Owner CHINA AGRI UNIV
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