Method for separating naphthoquinone active ingredients from sinkiang arnebia root

Abstract

The invention discloses a method for separating naphthoquinone active ingredients from sinkiang arnebia root, which comprises the following steps of: A, baking dried stems of the sinkiang arnebia root, performing ultrasonic extraction by using ethanol, and performing ultrasound so as to obtain dry extract; B, extracting ethanol dry extract by using solvents such as petroleum ether and ethyl acetate to prepare extract obtained by using ether and ethyl acetate; C, ultrasonically dissolving the extract by using the ethyl acetate that has equal volume to that of the extract, evaporating to dryness until the smell of ethyl acetate does not exist, connecting serially connected columns onto a middle-low pressure preparation type chromatographic column; D, eluting the columns under a screen monitoring figure of the middle-low pressure preparation type chromatographic column by using the petroleum ether as an eluant,, and collecting flowing fractions so as to obtain compounds; E, changing two serially connected columns on a middle-low pressure preparation type chromatography into a C18 column, dissolving the compounds with methanol, and processing the dissolved compounds by using the C18 column so as to purify the compounds; and F, subjecting the compounds to an alkaline reaction to obtain a purple colour and subjecting the compounds to a leukomethylene colour test to obtain a positiveresult. The method is easy to operate and the operation is easy and simple. The middle-low pressure preparation chromatography is used in the method; the method is an optimum technology for separating anticancer active ingredients efficiently; furthermore, the content of the separated naphthoquinone active ingredients is greater than 95 %.

Description

technical field [0001] The present invention relates to the technical field of extracting and separating naphthoquinones from medicinal plants, and more specifically relates to a method for extracting naphthoquinones, an antitumor active ingredient from comfrey, these active ingredients not only have antitumor effects, but also have Anti-inflammatory, antipyretic, analgesic, sedative, anti-pathogenic microorganisms, anti-fertility, liver protection, etc., can be widely used in the medical and health industry. Background technique [0002] Comfrey is the dried root of Xinjiang comfrey Arnebaieuchroma (Royle) Johnst, a plant of the family Boraginaceae. The main medicinal ingredients in comfrey are naphthoquinone pigments, including shikonin, deoxyshikonin, β, β-dimethylpropylene Acylshikonin, acetylshikonin, etc. The active ingredients of naphthoquinones are isolated from comfrey, and its pharmacological effects not only have anti-pathogenic microorganism effects, anti-virus ...

AI Technical Summary

Problems solved by technology

[0003] At present, there are many research reports on the extraction and purification methods of shikonadhiquinone components, but most of them are traditional extraction and separation processes, with long separation time and many extraction impurities; large solvent consumption; difficult to separate and obtain high-purity medicinal components