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Filter separator of mesenchyma stem cells and application thereof

A filter separator and quality stem cell technology, applied in biochemical instruments, biomass post-treatment, biomass pretreatment, etc., can solve the problems of increased chance of stem cell pollution, less stem cell harvest, and low survival rate of stem cells, so as to reduce pollution Occurrence, easy promotion and application, low price effect

Inactive Publication Date: 2013-04-17
GENERAL HOSPITAL OF THE SECOND ARTILLERY OF CHINESE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this mesenchymal stem cell isolation method are: ① time-consuming and laborious; ② low isolation efficiency; ③ low survival rate of stem cells; ④ less amount of harvested stem cells;

Method used

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  • Filter separator of mesenchyma stem cells and application thereof

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Embodiment 1

[0009] Installation and use of the present invention.

[0010] Mesenchymal stem cell filter separator consists of balloon 1, sterilizing filter (built-in 0.22μm filter membrane) 2, autoclavable rubber stopper 3, air compression chamber 4, conical stainless steel screen (100 mesh to 300 mesh ) 5. Sterilization filter (built-in 0.22 μm filter membrane) 6. Stem cell collection bottle 7 and pressure stabilizing port 8. Before use, the whole device can be divided into 3 parts: ①Hand squeeze the balloon 1; ②Connected sterilizing filter 2 and rubber plug 3; ③Connected parts other than ① and ②, including the air compression chamber 4 1. Conical stainless steel screen (100 mesh to 300 mesh) 5. Sterilization filter (built-in 0.22 μm filter membrane) 6. Stem cell collection bottle 7 and pressure regulator port 8. Parts ② and ③ were autoclaved (121°C, 30min). Under aseptic conditions, through aseptic operation, add the mixture of primary cells and tissues used to extract mesenchymal ste...

Embodiment 2

[0013] Human amniotic mesenchymal stem cells were isolated and prepared.

[0014] The filter separator for mesenchymal stem cells of the present invention is sterilized by high pressure steam (121° C., 30 min) before use. The full-term cesarean section placenta was taken under sterile conditions, and the amniotic membrane was bluntly peeled off to make the separated amniotic membrane as thin as possible. Take the fetal surface placental tissue, wash it repeatedly with PBS, cut it into fragments with a diameter of about 2mm with ophthalmic scissors, add 0.1% collagenase II, digest at 37°C for 50min, and then add it into the room through aseptic operation in the ultra-clean workbench. Install the mesenchymal stem cell filter separator in the conical stainless steel screen of the mesenchymal stem cell filter separator, inflate it with a balloon, collect the filtrate in the stem cell collection bottle, and then obtain purified Human amniotic mesenchymal stem cells.

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Abstract

The invention belongs to a medical apparatus and relates to a filter separator of mesenchyma stem cells and an application thereof. The filter separator of the mesenchyma stem cells is composed of an inflating bulb, a degerming filter, a rubber plug, an air compression room, a conical stainless steel screening net, a stem cell collecting bottle and a stable-pressure opening. The filter separator of the mesenchyma stem cells has the main characteristics and advantages as follows: (1) all parts can be conveniently detached, washed and sterilized; (2) due to the unique design of the conical cellscreening net, the surface tension of an upper fluid is reduced, the surface area, the volume and the lower-layer pressure of the bearing liquid are increased, the liquid can favorably flow down and the filtering efficiency of the mesenchyma stem cells is obviously increased; (3) the operation time of separating and preparing the mesenchyma stem cells is shortened, the possibility of polluting isreduced and the survival rate of the mesenchyma stem cells is increased; (4) the processing amount is great, the number of the separated and prepared mesenchyma stem cells is large and the quality ofthe separated and prepared mesenchyma stem cells is good; (5) the filter separator is convenient to operate and low in price. The filter separator provided by the invention can be used for separatingand preparing the mesenchyma stem cells from human bodies or animal placentas, amnions, umbilical cords and the like and the separated and prepared mesenchyma stem cells can be used for clinical treatment or laboratory research.

Description

technical field [0001] The invention belongs to medical devices, and in particular relates to a device for digesting, separating and collecting mesenchymal stem cells from human or animal placenta, amniotic membrane, umbilical cord and other tissues and its application method. Background technique [0002] With the in-depth development of the research and application of stem cells and tissue engineering technology, the separation and preparation of stem cells, as one of the key technologies in this field, has attracted increasing attention. So far, the separation devices for white blood cells and tumor cells used in the medical field have developed rapidly, but there are few separation devices for mesenchymal stem cells. Currently, the most widely used mesenchymal stem cell separation devices are cell screens, which are generally selected to be 100-mesh. The 300-mesh stainless steel screen is used to separate cells from digested tissue blocks by shaking, stirring and shaking...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/12
CPCC12M47/04
Inventor 王佃亮张艳梅孙晋伟
Owner GENERAL HOSPITAL OF THE SECOND ARTILLERY OF CHINESE PLA
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