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Method for quickly estimating specificity of influenza A virus host

A type of influenza A virus, host-specific technology, applied in the preparation of test samples, measuring devices, material analysis by electromagnetic means, etc., can solve the problems of incomplete evaluation of influenza A virus pathogenic variation and host specificity , to achieve the effect of adapting to large-scale quarantine, simple separation and purification, and high-throughput detection of viruses

Inactive Publication Date: 2013-09-25
NORTHWEST UNIV
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0006] In order to solve the technical problem that conventional methods for detecting avian influenza virus and detecting viral nucleic acid cannot fully evaluate the variation of influenza A virus pathogens and the specificity of the host, the present invention provides a method for rapidly evaluating the host specificity of influenza A virus

Method used

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  • Method for quickly estimating specificity of influenza A virus host

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Embodiment Construction

[0063] Realize the experimental material of the present invention specifically as follows:

[0064] 1), main materials and reagents:

[0065] Avian influenza virus was provided by Harbin Veterinary Research Institute; self-made hydroxylated magnetic particles;

[0066] 3'-N-Acetylneuraminyl-N-acetyllactosamine sodium salt(α-NeuNAc-(2→3)-β-D-Gal-(1→4)-D-GlcNAc) or 3'-Sialyllactose(α-NeuNAc-( 2→3)-β-D-Gal-(1→4)-D-Glc), 6'-Sialyllactose sodium salt(α-NeuNAc-(2→6)-β-D-Gal-(1→4) -D-Glc), phenylmethylsulfonphthalein fluoride (PMSF), ethanolamine, ProteinMarker, sigma; other commonly used reagents are of domestic analytical grade.

[0067] 2), the formula of main reagent:

[0068] Coupling buffer: 0.2mol / L pH5.4 acetic acid sodium acetate buffer;

[0069] Binding buffer: 20mmol / L Tris-HCl, 0.5mol / L NaCl, 10mmol / L CaCl 2 , 6mmol / L MnCl 2 , pH7.2;

[0070] Cleaning solution: 20mmol / L Tris-HCl, 0.5mol / L NaCl, 0.05% Tween20, pH7.2;

[0071] Elution buffer: 0.5% SDS;

[0072] Pre...

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Abstract

The invention relates to a method for quickly estimating specificity of an influenza A virus host. The method comprises the following steps of: primarily purifying the hemagglutinin of influenza A virus and preparing a sugar chain magnetic particle complex; combining the primarily purified hemagglutinin of the influenza A virus with the sugar chain magnetic particle complex; eluting the hemagglutinin of the influenza A virus, which is combined with the sugar chain magnetic particle complex; and identifying the eluted hemagglutinin of the influenza A virus so as to acquire the result of the specificity of the influenza A virus host and judge whether the influenza virus infects human bodies. The technical problem that the mutation of the pathogen of the influenza A virus and the specificity of the host of the influenza A virus cannot be completely estimated by the conventional influenza virus detecting means and virus nucleic acid detecting methods is solved. The method has the advantages that the method is quick, simple and convenient, has high efficiency and the like.

Description

technical field [0001] The invention relates to the field of influenza A virus, in particular to a method for evaluating host specificity of influenza A virus. Background technique [0002] Influenza A viruses are common influenza viruses and are prone to mutations. Avian influenza virus is a subtype of influenza A virus. Avian influenza is an acute infectious disease caused by avian influenza virus, which can also infect humans. Avian influenza virus not only seriously endangers the production of animal husbandry, especially the highly pathogenic H5N1 avian influenza virus has crossed the species barrier and directly caused human infection and death of the infected person. [0003] There are many types of avian influenza virus antigens and frequent mutations, making the prevention and control of highly pathogenic avian influenza very difficult. Therefore, it is necessary to develop new technologies that can be used to monitor and evaluate the variation and host specificit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N1/28G01N1/34G01N1/42G01N21/64
Inventor 李铮孙宇杜亚蓉祁会彩邓炜娜王秀荣杨刚龙
Owner NORTHWEST UNIV
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