Transgenic method for curcuma soloensis Voel.

A Solomon turmeric and transgenic technology, applied in the transgenic field of Solomon turmeric, can solve the problems of high cost, no successful report on the transgenic technology of ginger flowers, time-consuming and labor-intensive, etc., and achieves low cost, short cycle and high repeatability. Effect

Inactive Publication Date: 2013-05-22
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The latter two methods have many disadvantages: for example, they require an in vitro culture stage, require professional technicians to operate aseptically in the laboratory, time-consuming and labor-intensive, and require advanced professional experimental equipment and many tissue culture agents, cost expensive
[0008] At present, there is no successful report on the transgenic technology of Zingiberaceae flowers

Method used

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  • Transgenic method for curcuma soloensis Voel.

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) The pCAMBIA-1301 plasmid was extracted from Escherichia coli DH5α containing the pCAMBIA-1301 plasmid by conventional alkaline lysis method, and then diluted to 300 μg / mL with TE solution. This plasmid carries cauliflower mosaic virus (CaMV) 35S promoter and GUS reporter gene, and can be purchased from Bioreagent Company.

[0034] (2) Preparation of Solomon turmeric flowering plants: the Solomon turmeric that is planted in the ground is applied compound fertilizer 1 time every half a month, and the compound fertilizer is selected from N: P: K=1: 1-2: 1-2, and watered 1 time in the morning and evening every day. Without shade to ensure sufficient light, Solomon turmeric can bloom continuously from April to October, thereby increasing the quantity and quality of seeds;

[0035] (3) Artificial pollination of Solomon turmeric: observe the opening degree of the yellow florets of Solomon turmeric in August, select the florets whose petals are fully opened, scrape off the ...

Embodiment 2

[0039] (1) The pCAMBIA-1301 plasmid in this example is the same as that in Example 1, and the pCAMBIA-1301 plasmid is diluted to 500 μg / mL with TE solution.

[0040] (2) Preparation of Solomon turmeric flowering plants: the Solomon turmeric that is planted in the ground is applied compound fertilizer 1 time every half a month, and the compound fertilizer is selected from N: P: K=1: 1-2: 1-2, and watered 1 time in the morning and evening every day. Without shade to ensure sufficient light, Solomon turmeric can bloom continuously from April to October, thereby increasing the quantity and quality of seeds;

[0041] (3) Artificial pollination of Solomon turmeric: observe the opening degree of the yellow florets of Solomon turmeric in September, select the florets whose petals are fully opened, scrape off the pollen with tweezers, and smear the pollen on the stigma of another floret whose stamens have been removed;

[0042] (4) Inject 6 μL of 500 μg / mL pCAMBIA-1301 plasmid solution...

Embodiment 3

[0045] (1) The pCAMBIA-1301 plasmid in this example is the same as that in Example 1, and the pCAMBIA-1301 plasmid is diluted to 400 μg / mL with TE solution.

[0046] (2) Preparation of Solomon turmeric flowering plants: the Solomon turmeric that is planted in the ground is applied compound fertilizer 1 time every half a month, and the compound fertilizer is selected from N: P: K=1: 1-2: 1-2, and watered 1 time in the morning and evening every day. Without shade to ensure sufficient light, Solomon turmeric can bloom continuously from April to October, thereby increasing the quantity and quality of seeds;

[0047] (3) Artificial pollination of Solomon turmeric: observe the opening degree of the yellow florets of Solomon turmeric in July, select the florets whose petals are fully opened, scrape off the pollen with tweezers, and smear the pollen on the stigma of another floret whose stamens have been removed;

[0048] (4) With the flower 3 hours after cross-pollination in step (3)...

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Abstract

The invention discloses a transgenic method for Curcuma soloensis Voel. The method is fast and simple, and has high conversion efficiency, comprising the following steps: a) within 1 hour to 3 hours after Curcuma soloensis Voel has been cross-pollinated, injecting 3 to 10 mu L of a plasmid solution having a concentration of 300 to 500 mu g / mL into column caps of the Curcuma soloensis Voel, allowing the Curcuma soloensis Voel to grow and bear fruits, and harvesting seeds; b) culturing the harvested seeds, allowing seedlings to emerge from the seeds, detecting the plants through screening markson plasmids or by PCR molecular detection method, identifying positive plants with transferred plasmids, the positive plants being transgenic plants. The transgenic method provided in the invention marks the pioneering success of utilization of transgenic method in Zingiberaceae plants. The method is easy to operate, has low cost and high conversion rate, and can be applied to plant Curcuma soloensis Voel in large farmland in large scale. With the utilization of the method, requirement for an ornamental property of improved flowers can be satisfied, novel species of transgenic flowers can be supplied for flower market, ornamental value of Curcuma soloensis Voel can fully achieved, and aids for enterprises to improve market competitiveness and obtain high economic benefits are realized.

Description

Technical field: [0001] The invention relates to a plant transgenic method, in particular to a Solomon turmeric transgenic method. Background technique: [0002] Solomon turmeric (Curcuma soloensis Voel.) is a rare tropical ornamental flower belonging to the genus Curcuma in the family Zingiberaceae. It is a perennial bulbous herbaceous flower. It is native to the Solomon Islands and has been introduced and cultivated in Guangdong, my country. [0003] The plant height of Solomon turmeric is 30-60 cm. The plants are clustered, and the leaves are oblong and bright green. The flowering period is from April to October every year. Cylindrical spikes are drawn from the rhizomes, and the inflorescences are shaped like pagodas; the purple-red bracts are arranged tightly on the inflorescences, and the texture is as elegant as jade, gorgeous and colorful, and can be used as flower borders, Flower bed arrangement; in addition, Solomon turmeric is also an elegant flower material for ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/89C12N15/52C12N15/65C12N15/82
Inventor 吴国江张施君刘念盛爱武
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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