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Method for asexual propagation of wild aweto fungi

A technique of asexual reproduction of wild Cordyceps sinensis, applied in botany equipment and methods, fertilizer mixtures, horticulture, etc., can solve the problems of susceptibility to mold, bacterial contamination, unstable product quality, poor strain activity, etc. The effect of no variation and stable strain quality

Inactive Publication Date: 2011-09-21
杜保华
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for asexual reproduction of wild Cordyceps sinensis strains, to solve the poor activity of the prior art strains, susceptible to mold, bacterial contamination, resulting in unstable product quality

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Embodiment Construction

[0010] The specific implementation steps of the technical solution of the present invention are as follows:

[0011] 1. Select live cells: obtain wild strains from wild Cordyceps sinensis grasslands in Qinghai, Tibet, etc., and inoculate them under sterile conditions. The proportion of the medium used is: water 970, potato 310, peptone 5, potassium dihydrogen phosphate 4, Magnesium sulfate 2, agar 20, glucose 18, pH 6.

[0012] 2. Domesticated strains: Reduce the oxygen content in the incubator by 20%, the temperature is 19°C, and the humidity is 60%, simulating the natural environment of wild strains, and the growth period is about 15 days.

[0013] 3. Purification: Remove the resistance points of weak and small strains, and retain the hyphae with high activity and normal growth. The growth period is about 30 days.

[0014] 4. Rejuvenation: transfer the purified mycelia 2-3 times, and the growth period is 40 days to obtain excellent mother species of Cordyceps.

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Abstract

The invention provides a method for asexual propagation of wild aweto fungi and belongs to the technical field of biology. In the method, living cells of wild aweto are used as strains, and the aweto fungi suitable for artificial culture are obtained in a special potato dextrose agar (PDA) culture medium by a cloning technology; and the PDA culture medium comprises the following components by mass: 950 to 1,050 grams of water, 280 to 320 grams of potatoes, 4 to 8 grams of peptone, 3 to 5 grams of potassium dihydrogen phosphate, 1 to 2 grams of magnesium sulfate, 15 to 20 grams of agar and 18 to 23 grams of glucose, and the pH value is between 6.0 and 7.0. The method comprises the following steps: 1, selection of living cells; 2, domestication of strains; 3, purification; and 4, rejuvenation and obtaining of excellent mother seeds of aweto. The method has the advantages of reasonable nutrients of the PDA strain culture medium, high strain activity, no pollution by molds and bacteria, stable quality of fungi and no mutation of fungi. In addition, the content of effective nutrients of the aweto is high, and the good-quality and high-yield fruit body is obtained.

Description

technical field [0001] The invention belongs to the field of biotechnology. Background technique [0002] At present, most of the artificial propagation of Cordyceps sinensis adopts artificially cultivated Cordyceps for tissue separation. The problems in this method are: 1. The bacterial strains have poor activity and are susceptible to mold and bacterial contamination, resulting in unstable product quality. 2. The mutation speed is fast, resulting in the low effective nutritional content of Cordyceps, and in serious cases, it will lead to weak plants, diseased plants, or even extinction. 3. The nutrient composition of the strain PDA medium is low, and the C:N ratio is unreasonable. Contents of the invention [0003] The purpose of the present invention is to provide a method for the asexual reproduction of wild Cordyceps sinensis strains to solve the problem of poor activity of the strains in the prior art, which are easily polluted by molds and bacteria, resulting in un...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 杜保华
Owner 杜保华
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