Qualitative semi-quantitative dual-purpose female ovulation hormone detection test paper and colorimetric card

Abstract

The invention discloses a test paper for qualitative semi-quantitative dual-purpose detection of female ovulation, which is formed mainly by overlapping and connecting a bottom plate, a nitrocellulose membrane, an absorption pad, a polyester film and a glass fiber membrane mutually, wherein the middle of the bottom plate is adhered with the nitrocellulose membrane on which two antibody lines are engraved, one line is a detection line coated by a first anti-LH monoclonal antibody, and the other line is a quality control line coated by goat anti mouse IgG monoclonal antibody; and the upper edge position of the nitrocellulose membrane is adhered with the water absorption pad, the lower edge position of the nitrocellulose membrane is adhered with a combined pad which combines another anti-LH monoclonal antibody of marking colloidal gold or color rubber latex, and the lower edge position of the combined pad is adhered with a sample pad. A woman can use the test paper of the invention at home to qualitatively and semi-quantitatively detect the content of LH luteinizing hormone of the urine or blood of the woman, and knows the ovulation date, or knows whether hormonal infertility exists or not.

Description

technical field [0001] The present invention relates to a test paper for semi-quantitatively detecting female ovulation hormone levels and a method for using the test paper to detect ovulation, determine ovulation date or detect hormonal infertility. Background technique [0002] Luteinizing hormone (LH) is secreted by basophils of the pituitary gland. In women (LH) works in conjunction with FSH (follicle-stimulating hormone) to maintain the menstrual cycle of the ovaries, leading to ovulation and corpus luteum formation. The production of LH is controlled by the hypothalamic gonadotropin-releasing hormone, and is also regulated by positive and negative feedback from the ovary. Promoting ovulation Under the synergistic action of FSH, the corpus luteum is formed and progesterone is secreted, which can predict ovulation. When the follicle matures, it secretes a large amount of estrogen, which causes the pituitary gland to release a large amount of luteinizing hormone, formin...

AI Technical Summary

Problems solved by technology

[0006] At present, hospitals at home and abroad are using LH radioimmunoassay kits, LH enzyme immunoassay kits and qualitative colloidal gold test paper, some of which require hospital professionals and professional equipment to detect, and it takes a long time and is very complicated, so it cannot be used at home. Self-test, some can only be qualitative, can not play a guiding role

[0007] The CN00112779.9 patent of Kunming Yunda discloses a test paper for infertility detection and its detection method. It has only one antibody line on the nitrocellulose membrane and no quality control line. Misjudged infertility or low hormone levels

[0008] The patent CN200610200333 of Lanzhou University in Gansu discloses a rapid immunodiagnostic test strip of colloidal gold semi-quantitative method. The detection line and quantitative line are arranged left and right, on the same horizontal line, and a narrow seam is added in the middle, which is not convenient for batch production. Production

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