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Animal model constructing method for studying epithelial and stromal hyalinosis of hepatocellular carcinoma

An animal model, hepatocellular carcinoma technology, applied in the field of an ideal verification platform for the development of targeted anti-tumor drugs, can solve the problems of epithelial-mesenchymal-like changes, difficult to observe, etc., and achieves high success rate, wide popularization and application, and simple production process easy effect

Inactive Publication Date: 2011-09-14
ZHONGSHAN HOSPITAL FUDAN UNIV
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Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the epithelial-mesenchymal changes that occur in the process of tumor cell invasion and metastasis are difficult to observe, and to develop a simple and easy-to-use, widely popularized and applied, high success rate that can truly simulate the clinical in situ liver cancer. A method for constructing an animal model of the dynamic process of epithelial-mesenchymal transformation in transplanted tumors

Method used

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  • Animal model constructing method for studying epithelial and stromal hyalinosis of hepatocellular carcinoma
  • Animal model constructing method for studying epithelial and stromal hyalinosis of hepatocellular carcinoma
  • Animal model constructing method for studying epithelial and stromal hyalinosis of hepatocellular carcinoma

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Embodiment 1

[0028] (1) Take BALB / c nude mice (4-6 weeks, male) as experimental animals, each group of 5 nude mice, use 1.5% (1.5g dissolved in 100ml double distilled water) sodium pentobarbital (40mg / kg , Sinopharm product, item number B1202) intraperitoneal injection anesthesia;

[0029] (2) Mix the Matrigel matrix membrane (product of BD company, product number 354234) and serum-free high-sugar DMEM (product of GIBICO company product number 11995081) at a volume ratio of 1:1, and use the above-mentioned mixed solution to separate 8×10 6 A logarithmic growth stage liver cancer cell HCCLM3-control (HCCLM3 cells were transfected with a lentiviral empty vector. HCCLM3 cells were constructed, preserved and publicly used by the Liver Cancer Institute of Fudan University; the lentiviral empty vector was a product of Shanghai Jikai Gene Chemical Technology Co., Ltd. Product number GC080357), shRNA-CD151-HCCLM3 (HCCLM3 cells were transfected with lentivirus that interferes with CD151 expression plas...

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Abstract

The invention provides an animal model constructing method for studying epithelial and stromal hyalinosis of hepatocellular carcinoma. The method is characterized by the following steps: inoculating cancer cells to liver of an experimental animal in situ under guidance of ultrasonic equipment; and dynamically observing related index changes and lung metastasis conditions of epithelial and stromal hyalinosis. The method has the advantages that: the manufacturing process is simple and easy, has high success rate, and can be widely popularized and applied; and the manufactured animal mode can truly simulate the pathological process of clinical metastasis of hepatic carcinoma, and can dynamically reflect the developing process of the epithelial and stromal hyalinosis of the hepatic carcinoma.

Description

Technical field [0001] The present invention relates to constructing an animal model for studying the dynamic process of hepatocellular carcinoma epithelial mesenchymal transformation. The present invention can also become an ideal verification platform for the development of targeted anti-tumor drugs for the hepatocellular carcinoma epithelial mesenchymal transformation process. Background technique [0002] Primary liver cancer is one of the highly malignant tumors, and its mortality rate ranks second among malignant tumors in my country. Most deaths of liver cancer patients are related to liver cancer recurrence and metastasis. Studies have shown that the recurrence and metastasis of liver cancer is a complex process involving multiple genes and multiple steps, including tumor cell adhesion, migration, and tumor cell degradation of extracellular matrix. At present, the molecular mechanism of its occurrence is not fully understood. Further research The molecular mechanism of li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00A61K49/00G01N1/30G01N33/48A01K67/027
Inventor 樊嘉周俭柯爱武施国明黄晓勇
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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