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Method for extracting oligomeric proanthocyanidins from grape seeds

A technology of proanthocyanidins and grape seeds, which is applied in the fields of organic chemistry, reverse osmosis, ultrafiltration, etc., can solve the problems of product purity and yield needing further research, and achieve the goal of ensuring purity and yield, ensuring safety, and reducing extraction costs. Effect

Inactive Publication Date: 2011-08-24
JIANGSU XINGCHI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The shortcomings of the organic solvent method are: first, only the low-polymeric proanthocyanidins in the plant body can be extracted, while most of the high-polymeric proanthocyanidins remain in the plant body; second, the product purity is low, only about 50%-70%; Third, there are organic solvents remaining in the product to reduce safety, and the consumption of organic solvents is large, the cost is high, and waste water is easily generated
[0004]The second is water extraction method to extract proanthocyanidins, such as Chinese patent application number ZL02115423.6, water is selected as the extraction agent, the extraction time is long, the temperature is high, and it is easy to cause proanthocyanidin At the same time, the polarity of water is relatively large, and there are more dissolved impurities
However, the supercritical CO? extraction method is only in the laboratory stage, and its cost is high, so it is still difficult to realize industrialization
[0006] The fourth is the microwave extraction method, such as Chinese patent application number CN00120353.3, which is superior to the traditional water extraction method, but this is limited to the rough extraction stage of proanthocyanidins, and the final product purity The yield rate needs to be further studied

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A) To prepare the extract, first crush the clean and sun-dried dried grape seeds into 50-mesh small particles, and put them into the extraction kettle, then introduce hot water (distilled water) into the extraction kettle for circular extraction, and crush the small particles The weight ratio to distilled water at a temperature of 80°C is 1:50, the extraction pressure of the extraction kettle is controlled to 10MPa, the extraction temperature and extraction time are respectively controlled to 130°C and 30min, and the extraction is circulated twice to obtain the extract;

[0025] B) To prepare pure filtrate, first filter the extract through a 60-mesh stainless steel filter to obtain a crude filtrate, and then pass the crude filtrate through an ultrafiltration membrane, which is a polyvinylidene fluoride membrane with a pore size of 0.001 μm. The inlet pressure is 0.2MPa, the outlet pressure is 0.1MPa, the temperature and flow rate of the crude filtrate are controlled ...

Embodiment 2

[0028] A) To prepare the extract, first crush the clean and sun-dried dried grape seeds into 80-mesh small particles, and put them into the extraction kettle, and then introduce hot water (pure water) at a temperature of 98°C into the extraction kettle for extraction. Circular extraction, the weight ratio of crushed small particles to pure water at a temperature of 98°C is 1:30, the extraction pressure of the extraction kettle is controlled at 10MPa, the extraction temperature and extraction time are respectively controlled at 130°C and 30min, and the cyclic extraction is performed four times to obtain Extraction solution;

[0029] B) To prepare pure filtrate, first filter the extract through an 80-mesh stainless steel filter to obtain a crude filtrate, then filter the crude filtrate through an ultrafiltration membrane, the ultrafiltration membrane is a polyvinylidene fluoride membrane with a pore size of 0.02 μm, and the ultrafiltration membrane The inlet pressure is 0.5M...

Embodiment 3

[0032] A) To prepare the extract, first crush the clean and sun-dried dried grape seeds into 65-mesh small particles, and put them into the extraction kettle, then introduce hot water (distilled water) at a temperature of 70°C into the extraction kettle for circulation For extraction, the weight ratio of crushed small particles to distilled water at a temperature of 70°C is 1:40, the extraction pressure of the extraction kettle is controlled to 12.5MPa, the extraction temperature and extraction time are controlled to 180°C and 15min respectively, and the extraction is circulated three times to obtain the extract ;

[0033] B) To prepare pure filtrate, first filter the extract through a 40-mesh stainless steel filter to obtain a crude filtrate, then filter the crude filtrate through an ultrafiltration membrane, the ultrafiltration membrane is a polyvinylidene fluoride membrane with a pore size of 0.01 μm, and the ultrafiltration membrane The inlet pressure is 0.35MPa, the o...

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Abstract

The invention discloses a method for extracting oligomeric proanthocyanidins from grape seeds, and belongs to the field of the separation and purification of the oligomeric proanthocyanidins. The method comprises the following steps of: preparing extracting solution, namely crushing dried grape seeds into small particles, loading the small particles into an extraction kettle, adding hot water into the kettle for circular extraction, controlling the size of the small particles and the weight ratio of the small particles to the water, controlling the extraction pressure, temperature and time of the extraction kettle, and controlling the times of circular extraction to obtain the extracting solution; preparing pure filtrate, namely filtering the extracting solution through a filter screen to obtain crude filtrate, filtering the crude filtrate through an ultrafiltration membrane, controlling the inlet pressure and outlet pressure, temperature and flow velocity of the ultrafiltration membrane to obtain fine filtrate, performing reverse osmosis filtration on the fine filtrate, and collecting generated filtrate for recycling; and concentrating and drying, namely adding the pure filtrate into a concentration drying tank for concentration and drying, and controlling the temperature, pressure and time of concentration and drying of the concentration drying tank to obtain an oligomeric proanthocyanidins product. The method has the advantages that: extraction cost is reduced, and the safety of the product is guaranteed; energy sources are saved, and the purity and yield of the product are guaranteed; and pollution-free production and environmental protection are embodied.

Description

technical field [0001] The invention relates to a method for extracting proanthocyanidins from grape seeds, belonging to the technical field of separation and purification of proanthocyanidins . Background technique [0002] Proanthocyanidins (grape seed extract), referred to as OPC, is a bioflavonoid with a special molecular structure. It is currently recognized internationally as the most effective natural antioxidant for eliminating free radicals in the human body. It is the most powerful found so far. A free radical scavenger with very strong in vivo activity. Experiments have proved that OPC's ability to resist free radical oxidation is 50 times that of vitamin E and 20 times that of vitamin C, and it can be absorbed quickly and completely. It can reach the highest blood concentration in 20 minutes after oral administration, and its metabolic half-life is as long as 7 hours. Structurally, proanthocyanidins are composed of different amounts of catechin or epicatechin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62B01D61/14B01D61/02
Inventor 张慧东陈伟卜英俊顾培华
Owner JIANGSU XINGCHI BIOTECH
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