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Application of astragaloside II in preparing anti-cancer medicines

A technology of astragalus saponin and anti-liver cancer, which is applied in the direction of drug combination, antineoplastic drugs, and pharmaceutical formulations, and can solve the problems of complex ingredients, control, difficult quality and stability, etc.

Active Publication Date: 2012-09-26
CHIATAI QINGCHUNBAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In addition, total astragalus saponins or astragalus injection are also complex in composition, making it difficult to control their quality and stability so that they can meet the requirements of modern medication

Method used

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  • Application of astragaloside II in preparing anti-cancer medicines
  • Application of astragaloside II in preparing anti-cancer medicines
  • Application of astragaloside II in preparing anti-cancer medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Preparation of Astragaloside II and IV

[0055] 1. Experimental materials

[0056] 1.1 Astragalus membranaceus test drug (origin in Gansu, purchased from SDIC Pharmaceutical Anhui Co., Ltd., batch number: 080623; identified as Astragalus membranaceus by Professor Liu Shoujin from the Teaching and Research Office of Medicinal Botany, Anhui College of Traditional Chinese Medicine); Product Testing Institute, batch number: 0781-200210); astragaloside Ⅱ standard product (provided by Shanghai Traditional Chinese Medicine Standardization Research Center, batch number: 07-1015); silica gel high-efficiency G plate (Qingdao Ocean Chemical Factory); silica gel for column chromatography (Qingdao Ocean Chemical Industry Group); D101 resin (Tianjin Youchang Industry and Trade Co., Ltd.); ethanol, n-butanol, ethyl acetate, and methanol were analytically pure, acetonitrile was chromatographically pure, and water was double-distilled water.

[0057] 1.2 Instruments

[0058] A...

Embodiment 2

[0121] Example 2 Study on Antitumor Effects of Astragalus Saponins Ⅱ and Ⅳ

[0122] 1. Experimental materials

[0123] 1.1. Cell lines

[0124] Human liver cancer cell line BEL-7402 and liver cancer drug-resistant cell line BEL-7402 / 5-FU were purchased from Nanjing Kaiji Biotechnology Development Co., Ltd.

[0125] 1.2 Drugs and reagents

[0126] Astragaloside Ⅱ and Astragaloside Ⅳ (ASⅡ, ASⅣ) (Laboratory self-made)

[0127] D-verapamil (R(+)-VERAPAMIL HCL, VRP) was purchased from IL Company, USA, with a purity of >99%

[0128] 5-FU Tianjin Jinyao Amino Acid Co., Ltd., batch number 0810211

[0129] MMC Zhejiang Hisun Pharmaceutical Co., Ltd., batch number 0810211

[0130] ADR Zhejiang Hisun Pharmaceutical Co., Ltd., batch number 0810211

[0131] DMEM and fetal bovine serum American Gibco products

[0132] MTT American Sigma products

[0133] DMSO American Sigma products

[0134] Rho123 Product of Sigma, USA

[0135] Trypsin BIOSHARP products

[0136] PBS Boster Biolo...

Embodiment 3

[0272] Tablet: Astragaloside Ⅱ 10mg

[0273] Lactose 187mg

[0274] Corn starch 50mg

[0275] Magnesium Stearate 3mg

[0276] Preparation method: mix the active ingredient, lactose and starch, moisten it evenly with water, sieve the wetted mixture and dry it, then sieve it, add magnesium stearate, then press the mixture into tablets, each tablet weighs 250mg, the active ingredient content 10mg.

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Abstract

The invention relates to technical fields of traditional Chinese medicine active ingredient extraction and application. The astragaloside II has obvious inhibition effect on proliferation of two human liver cancer cells, namely BEL-7402 and BEL-7402 / 5-FU under the condition of different concentrations, and can be administrated with other medicines simultaneously or sequentially to achieve the anti-tumor effect. The invention provides an anti-tumor medicine with definite response relationship and corroborant structural components, lays a foundation for developing new clinical anti-tumor medicines, provides a medicine for reversing multidrug resistance to achieve the anti-tumor effect, and provides a new scheme for solving the problem of multidrug resistance of chemotherapeutics.

Description

technical field [0001] The invention relates to the technical field of extraction and application of active ingredients of traditional Chinese medicines. It specifically relates to the application of astragaloside II in the preparation of anti-liver cancer drugs. Background technique [0002] In a normal body, cell apoptosis and cell proliferation are always under strict regulation to maintain a dynamic balance. Once this balance is broken, it will lead to the occurrence of many diseases. Due to the decline in the function of apoptosis, mutant cells cannot be effectively cleared and accumulate to form tumor. Drugs that can induce apoptosis play an important role in tumor therapy. [0003] Multidrug resistance (Mtltidrug Resistance, MDR) is the appearance of cross-resistance to multiple chemotherapeutic drugs with different structures and functions after tumor cells are induced by one drug. The mechanism of tumor MDR is extremely complex, which is the result of the compreh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61P35/00
Inventor 许杜娟王培培高建
Owner CHIATAI QINGCHUNBAO PHARMA
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