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Microcystin degrading strain and method for degrading MC-LR (microcystins-LR) by same

A technology of microcystin and MC-LR, which is applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of limitation, complicated extraction process, high price, etc., and achieve low cost, The effect of broad application prospects

Active Publication Date: 2011-08-17
溧阳常大技术转移中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex extraction process of MCs, the high cost of purification, and the high price of pure MCs, the price of a 10ug MC-LR imported standard product is as high as 3,500 yuan, which makes it possible to use MCs as the only source of carbon and nitrogen. The application of large-scale screening of MCs degrading bacteria is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The inoculated single strain was cultivated in inorganic salt medium with pH 5, 6, 7, 8, and 9 at 30°C at a shaker speed of 120 r / min, and the content of MC-LR was detected every 24 hours. The initial concentration of MC-LR was 286.75 ug / L, after 48 hours, when the pH was 5, the removal rate of MC-LR was 46.73%; when the pH was 6, the removal rate of MC-LR was 58.04%; when the pH When pH was 7, the concentration of remaining MC-LR in the inorganic salt medium was only 82.13 ug / L, and the degradation rate reached 71.36%. The strain had strong adaptability under neutral conditions; when the pH was 8, MC-LR The removal rate of LR was 61.87%; when the pH was 9, the removal rate of MC-LR was 53.84%.

Embodiment 2

[0040] Bacteria with mass fractions of 2%, 4%, 6%, 8%, and 10% were respectively inoculated in inorganic salt medium, cultured at 30°C at a shaker speed of 120 r / min, and MC- LR content. The initial concentration of MC-LR was 286.75 ug / L. After 48 hours, when the inoculation amount was 2%, the removal rate of MC-LR was 53.84%; when the inoculum amount was 4%, the removal rate of MC-LR was 61.87 %; when the inoculation amount was 6%, the removal rate of MC-LR was 65%; when the inoculum amount was 8%, the concentration of remaining MC-LR in the inorganic salt medium was only 86.14 ug / L, and the degradation rate reached 69.96%; when the inoculation amount was 10%, the removal rate of MC-LR was 66.95%.

Embodiment 3

[0042] Inoculate a single strain in an inorganic salt medium with a carbon source concentration of 0, 100, 200, 300, 400, and 500 mg / L, culture at 30°C at a shaker speed of 120 r / min, and detect MC every 24 hours - Content of LR. The initial concentration of MC-LR was 286.75 ug / L. After 48 hours, when no additional carbon source was added, the removal rate of MC-LR was 51.58%; when the concentration of additional carbon source was 100mg / L, the removal rate of MC-LR was 53.84%; when the added carbon source concentration was 200mg / L, the removal rate of MC-LR was 56.06%; when the added carbon source concentration was 300mg / L, the removal rate of MC-LR was 58.04%; when the added carbon source When the concentration was 400 mg / L, the removal rate of MC-LR was 63.64%; when the carbon source concentration was 500 mg / L, the concentration of remaining MC-LR in the inorganic salt medium was 105.01 ug / L, and the degradation rate reached 63.38 %, the addition of carbon source can promot...

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Abstract

The invention belongs to technical field of environmental-friendly water treatment and relates to an MC-LR (microcystin-LR) degrading strain screened from bottom sediments of streamsides with high blue-green algae pollution in the Taihu Lake and a method for degrading microcystins by the same. Currently, the strain is preserved in the China general microbiological culture collection center (CGMCC) and has a preservation number of CGMCC NO.4498. By identification, the strain is bacillus (bacillussp.). The method for degrading the microcystins by the MC-LR degrading strain comprises the following steps of: inoculating the MC-LR degrading strain into an inorganic salt culture medium which uses the MC-LR as the only carbon source and nitrogen source and has a pH value of 5 to 9 according to an inoculation rate of 2 to 10 mass percent; and cultivating the MC-LR degrading strain for 48 hours in a shaking table at a temperature of 30 DEG C under a rotating speed of 100 to 180 r / min so as to degrade the MC-LR. The stain is screened from a water body of the Taihu Lake, is suitable for the natural environment of the Taihu watershed and has wide application prospect for removing the MC-LR in the water body of the Taihu watershed by a biological method.

Description

technical field [0001] The invention belongs to the technical field of environmental protection water treatment, and in particular relates to a microcystin-LR (MC-LR) degrading bacterium screened from Taihu cyanobacteria heavily polluted riverside sediment and a method for degrading microcystin by using the same. Background technique [0002] With the continuous and rapid development of industry and agriculture in the Taihu Lake area, a large amount of wastewater containing nitrogen and phosphorus is discharged into natural water bodies, resulting in increasing eutrophication of water bodies and frequent outbreaks of cyanobacteria blooms. According to research reports, 25% to 70% of cyanobacterial bloom pollution can produce microcystins (MCs). MCs are a class of cyclic heptapeptide hepatotoxins with more than 80 isomers, among which MC-LR (molecular formula is C 49 h 75 N 13 o 12 ) and MC-RR (molecular formula is C 49 h 74 N 10 o 12 ) are two types of MCs with high ...

Claims

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Application Information

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IPC IPC(8): A62D101/28C12R1/07C12N1/20A62D3/02C02F3/34
Inventor 张文艺姚立荣李秋艳邱小兰范培成
Owner 溧阳常大技术转移中心有限公司
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