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Method for in vitro regeneration culture of true leaves of Chinese cabbage

A cultivation method and in vitro regeneration technology, applied in the field of agriculture, can solve the problems of long cultivation period and complicated cultivation procedures, and achieve the effects of low cost, simple cultivation method and large quantity

Inactive Publication Date: 2012-05-23
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method effectively solves the problems of complex in vitro regeneration culture procedure and long culture period of Chinese cabbage, and fills the blank of directly regenerating plants with stalked true leaves of Chinese cabbage.

Method used

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  • Method for in vitro regeneration culture of true leaves of Chinese cabbage
  • Method for in vitro regeneration culture of true leaves of Chinese cabbage
  • Method for in vitro regeneration culture of true leaves of Chinese cabbage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) Material

[0040] Orange Chinese cabbage 06J28 (references, Deng Yongling, Zhang Lugang*, Dong Meiyun, Fan Aili, Hui Maixia, Zhang Mingke; Histological observations on regeneration of adventitious buds from petioled cotyledons of Chinese cabbage, Northwest Agricultural Journal, 2008, 17( 4): 238~243).

[0041] 2) method

[0042] The first step, aseptic vaccine culture

[0043] Select orange Chinese cabbage 06J28 seeds with full grains, and under sterile conditions, first sterilize the surface with 70% alcohol by volume for about 0.5-1min, then sterilize with 3% NaClO solution for 13-15min, and then use sterile water repeatedly Rinse 4 to 5 times, 5 minutes each time. Finally, the seeds were sown on the surface of 1 / 2 MS basic medium, and cultivated under the conditions of a temperature of 25±1°C, a light time of 16h / d, and a light intensity of 2000lx;

[0044] The second step is to select plants suitable for seedlings

[0045] About 9 to 10 days after sowing...

Embodiment 2

[0071] 1) Material

[0072] Common Chinese cabbage 92S105 (same as 92-S 105 ) (References, Ke Guilan, Song Yanzhi, Zhao Limin, Zhang Lugang, etc., Qinbai No. 4 Chinese Cabbage Breeding. Shaanxi Agricultural Science, 1996, No.4, 12-15).

[0073] 2) method

[0074] The first step, aseptic vaccine culture

[0075] Select common Chinese cabbage 92S105 seeds with full grains. Under aseptic conditions, first sterilize the surface with 70% alcohol by volume for about 0.5-1min, then sterilize with 3% NaClO solution for 13-15min, and then use Rinse with sterile water 4 to 5 times, 5 minutes each time. Finally, the seeds were sown flat on the surface of the conventional 1 / 2 MS basic medium, and cultivated under the conditions of a temperature of 25±1°C, a light time of 16h / d, and a light intensity of 2000lx;

[0076] The second step is to select plants suitable for seedlings

[0077] After culturing for 9-10 days and the seedlings grow upright, select the plants that grow robus...

Embodiment 3

[0088] 1) Material

[0089] Orange Chinese cabbage 01S1024 (References: Zhang Lugang, Hui Maixia, Zhang Mingke, Breeding of a new colorful Chinese cabbage variety "Jinguan 2". Journal of Northwest Agricultural Science 2007, 16 (1): 204-206).

[0090] 2) method

[0091] The first step, aseptic vaccine culture

[0092] Select orange Chinese cabbage 01S1024 seeds with full grains, and under aseptic conditions, first sterilize the surface with 70% alcohol by volume for about 0.5-1min, then sterilize with 3% NaClO solution for 13-15min, and then use Rinse with sterile water 4 to 5 times, 5 minutes each time. Finally, the seeds were sown on the surface of conventional 1 / 2 MS basic medium, and cultivated under the conditions of temperature 25±1°C, light time 16h / d, light intensity 2000lx;

[0093] The second step is to select plants suitable for seedlings

[0094] After cultivating for 9-10 days, when the seedlings grow upright, select the plants that grow robustly, and the f...

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Abstract

The invention discloses a method for the in vitro regeneration culture of true leaves of Chinese cabbage. In the method, a true leaf is adopted as an explant for the first time on the basis of the traditional tissue culture of the Chinese cabbage, and an in vitro regeneration system of the true leaves of the Chinese cabbage is optimized in terms of appropriate seedling state, cutting mode, inoculation mode and hormone concentration. In the culture method, 6-benzylamino adenine (BA) and naphthylacetic acid (NAA) are only needed to be added into the conventional murashige and skoog (MS) basic culture medium; the method is simple and easy to operate; meanwhile, cultured adventitious buds are large in number, good in quality, robust and not abnormal, and have high regeneration rate and short regeneration period. The invention provides a novel way for obtaining a novel transgenic receptor material of the Chinese cabbage, and has important realistic significance for innovating the germplasmand novel variety of the Chinese cabbage by utilizing a modern genetic engineering technology.

Description

technical field [0001] The invention belongs to a cultivation method in the technical field of agriculture, in particular to a cultivation method for adventitious bud induction and plant regeneration using Chinese cabbage true leaves as explants. Background technique [0002] Chinese cabbage originated in my country and is planted in the north and south. It has a long history of cultivation and is an indispensable and important vegetable in people's life. It is delicious and nutritious. It is known as the "king of dishes" and is loved by the masses. favorite. [0003] The innovation of Chinese cabbage germplasm and new varieties through genetic engineering technology has the advantages of directness, rapidity and strong pertinence. Establishing an efficient and stable regeneration system is the prerequisite for the genetic transformation of Chinese cabbage. Compared with other crops of the Brassica genus, Chinese cabbage belongs to the AA genotype crop of the Brassicaceae, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张鲁刚刘学成茹磊李海萍惠麦侠张明科
Owner NORTHWEST A & F UNIV
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