Induced rapid propagation culture medium for somatic embryos of leaves in vitro of photinia x frasery

Abstract

The invention discloses an induced rapid propagation culture medium for somatic embryos of leaves in vitro of photinia x frasery, which comprises a bud starting culture medium, a test-tube seedling enrichment medium, a first somatic embryo and / or adventive bud formation culture medium, a second somatic embryo and / or adventive bud formation culture medium, a third somatic embryo and / or adventive bud formation culture medium, a first somatic embryo and / or adventive bud growth and differential medium, a second somatic embryo and / or adventive bud growth and differential medium, and a sound seedling rooting medium. By using the induced rapid propagation culture medium for the somatic embryos of the leaves in vitro of the photinia x frasery, a basal culture medium and content are improved, and the culture condition is adjusted to ensure that the photinia x frasery which is difficult to propagate propagates rapidly through the occurring pathway of the somatic embryos, the plant transplantation survival rate is over 95 percent, a seedling grows healthily, so that the problem of quality reduction of a good seed can be effectively solved, a large amount of high-quality purified and rejuvenated virus-free nursery stocks can be provided for production, and an ideal acceptor system can be provided for genetic transformation or mutation breeding seed selection of the photinia x frasery.

Description

technical field [0001] The invention relates to a culture medium for inducing rapid propagation of somatic embryos from in vitro leaves of Photinia fragrans, belonging to the field of botany. Background technique [0002] Photinia ( Photiniaⅹfrasery ) is a hybrid of Photinia glabra (P.glabra) and Photinia (P.serrulata), which belongs to the evergreen broad-leaved small trees or multi-branched shrubs of the genus Photinia in the Rosaceae family. It is widely distributed in Japan, the United States and Europe. Because its new shoots and young leaves are as bright as fire, bright and long-lasting, with strong germination after pruning and compact plant shape, it is often used as a high-grade ribbon in garden landscapes; or it can be cultivated as a single-stemmed, spherical crown, and planted alone in the green space; or As a street tree; or potted and placed in the porch or indoors, the effect is good. It is a garden plant with high ornamental value. [0003] Since Photinia ...

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Problems solved by technology

[0003]Since Photinia fragrans was introduced into China in the 1990s, it has been listed as a colorful tree species with high development value, but Photinia frondosa is different from many original Compared with tree species, the provenance in our country is scarce, and there has been no report of fruiting so far, and the excellent varieties introduced from abroad are also susceptible to fungal diseases such as leaf spot due to long-term cutting propagation, and they are also vulnerable to aphids in spring and summer. , the virus is serious, the leaf spots are easy to fall off, the ornamental value is reduced, and the degree of improved varieties is reduced; and the use of inferior seedlings leads to the loss of the inherent excellent characteristics of Photinia fragrans, which to a certain extent restricts the development and promotion of this tree species Therefore, the selection and breeding of excellent Photinia strains has become a very prominent production problem. Tissue culture and rapid propagation is an effective way for the industrialization of Photinia improved varieties, and there are many reports on related aspects, but the direct induction of Photinia leaves There are no successful reports on the technical research of somatic embryos and regenerative rapid propagation systems

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