Inverted digital holographic microscope

Abstract

The invention discloses an inverted digital holographic microscope, which belongs to the digital holographic technical field, and can be used for three-dimensional real-time appearance measurement and biological cell imaging. In the inverted digital holographic microscope, an optical fiber coupler 4 is arranged in front of a laser 5 and connected with an optical fiber beam splitter 3 through an optical fiber; the optical fiber beam splitter 3 is connected with two paths of optical fibers respectively connected with an optical fiber collimator 1 and an optical fiber collimator 6, a sample stage 11 for placing a sample 13 is arranged under the optical fiber collimator 1, and the sample stage 11 is connected with a two-dimensional translation stage 12; a micro objective mounted on a one-dimensional translation stage 10 is located under the sample stage 11, and the micro objective 9 and the optical fiber collimator 6 are aligned with two mutually vertical sides of a beam combination crystal 8; and a CCD (charge-coupled device) camera 7 is located under the beam combination crystal 8. The inverted digital holographic microscope can be used for observing the living cell which is located at the bottom of a culture dish and grows along a wall of the culture dish with a high resolution ratio for long time, and is highly integrated and small in volume; moreover, with the optical fiber connection, the laser can be freely mounted on other parts of the system.

Description

technical field [0001] The invention discloses an inverted digital holographic microscope, which belongs to the technical field of digital holography and can be used for three-dimensional real-time shape measurement and biological cell imaging. Background technique [0002] In recent years, the development of biomedicine has promoted the development of observation technology at the scale of biological cells. Traditional optical microscopes cannot measure the three-dimensional morphology of biological cells; while confocal microscopes have high resolution, but due to the need to calibrate biological samples, it will affect them, which is not conducive to the need for non-destructive observation. As a microscopic imaging technology, digital holography is non-destructive, real-time, and can obtain quantitative phase distribution, which is exactly its advantage in imaging biological samples. Living biological cells are generally transparent structures, so their phase images can...

AI Technical Summary

Problems solved by technology

[0003] In order to solve the technical problem that the digital holographic microscope has a large volume and weight, and adopts an upright structure, it is impossible to observe the living cells in the culture dish, the present invention proposes an inverted digital holographic microscope

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