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SNP composite detection system and detection method

A composite detection and system technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of small molecules, DNA molecule damage, STR locus can not be effectively typed, etc., to achieve good typing results, accurate results

Active Publication Date: 2011-07-06
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Abstract
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AI Technical Summary

Benefits of technology

This technology allows for high speed testing with many different types of DNA sequences simultaneously on one device called a combination detector or microscope plate (MCP). It also has several technical benefits such as accuracy during genetic sequencings, automatic processing ability after each test, easy interpretation of individual identities like BAO genes, and better quality typing even when sampled from low levels of contamination.

Problems solved by technology

This patents discuss how current techniques used for identifying individuals' sources during investigative material searches have limitations such as difficulty achieving effective resolution due to sample degenerations caused by environmental factors like heat, moisture, light, and other factors. These issues limit their effectiveness when analyzing large numbers of suspect samples containing multiple types of nucleic acid markers. Therefore there exists a challenge to create a detection systems capable of accurately distinguishing different forms of terrorism threatening biological events while also being able to identify specific ones among them quickly enough to provide powerful supporting services against criminals.

Method used

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  • SNP composite detection system and detection method

Examples

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Effect test

Embodiment 1

[0073] Embodiment 1, verification of the SNP composite detection system of the present invention

[0074] 1. Verification of the accuracy of the SNP composite detection system of the present invention

[0075] Using the SNP composite detection system of the present invention, according to the SNP composite detection method for individual identification, ABO genotyping and gender identification provided by the present invention, 200 Han nationality unrelated individual samples are detected to verify the SNP composite detection of the present invention system accuracy.

[0076] The SNP composite detection system includes 48 SNP sites, an amplification primer set, a micro-sequencing primer set, and a universal chip;

[0077] The set of amplification primers includes 48 pairs of amplification primers corresponding to the 48 SNP sites one-to-one, and each pair of amplification primers can amplify the mutant or wild type that includes its corresponding SNP site on the DNA to be det...

Embodiment 2

[0190] Embodiment 2, using the SNP composite detection system of the present invention to detect the paternity test material

[0191] By using the SNP composite detection system of the present invention to detect the venous blood DNA of 20 cases of triplet families (father-mother-son) whose genetic relationship has been determined, the method and steps are consistent with the first part of Example 1, and the paternity test results are consistent with routine use Identifiler Kit ( The results of the STR typing test using PCR Amplification Kit) were consistent, and no typing results that deviated from the Mendelian inheritance law were found, and all loci were compounded by the Mendelian inheritance law.

Embodiment 3

[0192] Embodiment 3, using the SNP composite detection system of the present invention to detect the normal inspection materials of individual identification cases

[0193] Use the SNP composite detection system of the present invention to inspect and detect 10 normal inspection materials (5 on-site blood samples and 5 suspect blood samples) collected in the daily inspection of 5 individual identification cases collected by the Material Evidence Appraisal Center of the Ministry of Public Security. And step is consistent with the first part of embodiment 1, simultaneously to scene blood sample and suspect blood sample use Identifiler kit ( PCR Amplification Kit) for STR typing detection. The results show that the on-site sample and the suspect sample get the same identification or exclusion result through STR typing test, and the same result can be obtained by using the SNP composite detection system of the present invention for normal sample.

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Abstract

The invention provides a single nucleotide polymorphism (SNP) composite detection system and an SNP composite detection method. The SNP composite detection system comprises a group of 48 SNP loci suitable for forensic medicine application, an amplification primer group, a mini-sequencing primer group and a universal chip, and can be used for individual identification, ABO genotyping and sex determination. The invention also provides the SNP composite detection method for the individual identification, the ABO genotyping and the sex determination by using the SNP composite detection system. Byamplifying nucleotide fragments containing the 48 SNP loci of deoxyribonucleic acid (DNA) of a sample to be detected, performing extension reaction on mini-sequencing primers and performing SNP locus-based genotyping on an amplification product by using the universal chip, the capacity of analyzing trace degraded sample DNA is improved, the sample range of a crime scene is expanded, and powerful technical support is provided for the criminal enforcement and administrative enforcement of public security organs.

Description

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Claims

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Application Information

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Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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