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Tissue engineering nerval stent and preparation method and application thereof

A tissue engineering and nerve technology, applied in the field of medical materials, can solve the problems of regeneration nerve conduction velocity, the number of axons and the thickness of myelin sheath, and the deterioration of functional recovery results, so as to increase the length of nerve defects, repair nerve defects, and improve repair speed. Effect

Inactive Publication Date: 2011-07-06
卢世璧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the repair experiments of defects with different lengths, it was found that with the increase of the length, the conduction velocity, number of axons, myelin sheath thickness and functional recovery of the regenerated nerves gradually deteriorated after CEAN repair.

Method used

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  • Tissue engineering nerval stent and preparation method and application thereof
  • Tissue engineering nerval stent and preparation method and application thereof
  • Tissue engineering nerval stent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Material source: Rat sciatic nerve.

[0048] 2. CEAN preparation process:

[0049] 1) Under sterile conditions, remove epineurium fat and connective tissue under a microscope;

[0050] 2) At 25°C, shake and soak in distilled water on a shaker for 24 hours;

[0051] 3) Shake, soak and rinse in the first extract for 24 hours (the preparation method of the first extract is as follows: dissolve SB-10 in 0.01M PBS solution to prepare a 125mM SB-10 solution);

[0052] 4) Rinse with 0.01M PBS solution for 30 minutes;

[0053] 5) Shake and wash in the second extract for 24 hours (the preparation method of the second extract is as follows: Dilute Triton X-200 with 0.01MPBS to a concentration of 0.14% (v / v, volume percentage content), and at the same time, SB-16 Dissolved in this solution, prepared as 0.6mM SB-16);

[0054] 6) Repeat steps 3), 4) and 5) in sequence once;

[0055] 7) Rinse with 0.01M PBS solution for 30 minutes;

[0056] 8) Soak in 2U / ml ChABC solution fo...

Embodiment 2

[0069] 1 Source of material: Human ulnar nerve (donor who died of acute craniocerebral injury).

[0070] 2 preparation process:

[0071] 1) Under sterile conditions, remove epineurium fat and connective tissue under a microscope;

[0072] 2) At 10°C, shake and soak in distilled water on a shaker for 24 hours;

[0073] 3) Shaking, soaking and rinsing in the first extract for 24 hours (the preparation method of the first extract is as follows: dissolve SB-10 in 0.01M PBS solution to prepare a 125mM SB-10 solution);

[0074] 4) Wash with 0.1M PBS solution for 40 minutes

[0075] 5) Shake and wash in the second extract 2 for 24 hours (the preparation method of the second extract is as follows: Dilute Triton X-200 with 0.01M PBS to a concentration of 0.14% (v / v), and dissolve SB-16 in it at the same time solution, prepared as 0.6mM SB-16);

[0076] 6) Repeat steps 3), 4) and 5) in sequence once;

[0077] 7) washing the human ulnar nerve with 0.01MPBS solution for 40 minutes; ...

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Abstract

The invention discloses a tissue engineering nerval stent and a preparation method and an application thereof. The chemical extraction method is adopted for removing main antigens, namely cells, axons and myelin sheaths, which can cause the rejection, and chondroitinase ABC is further adopted for removing chondroitin sulfate proteoglycan in xenogeneic nerves, thereby getting acellular xenogenic nerves with a complete vessel of a basilar membrane of an extracellular matrix and can be used as the tissue engineering nerval stent. The tissue engineering nerval stent is applicable to restoring peripheral nerve defect of rats, rabbits, dogs, human beings and the like, plays a very obvious role in promoting the regeneration of the axons and can further increase the length of restoring the peripheral nerve defect, thereby being a great nerve transplantation medical material.

Description

technical field [0001] The invention relates to medical materials, in particular to a chemically extracted acellular nerve (CEAN) and its application, and a preparation method for promoting the axonal growth of the chemically extracted acellular nerve and increasing its effect on repairing peripheral nerve defects. Background technique [0002] In mammals, the nerve tissue of adult individuals has limited regenerative capacity, and peripheral nerve injury and the resulting nerve defect after injury are common clinically disabling diseases. The current method for repairing peripheral nerve defects is autologous nerve transplantation as the standard operation, but the source of nerves in the donor area is limited. Chemically acellular allogeneic nerve (CEAN) is one of the ideal materials to replace autologous nerve transplantation. However, in the repair experiments of defects with different lengths, it was found that with the increase of the length, the conduction velocity, ...

Claims

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Application Information

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IPC IPC(8): A61L27/36
Inventor 卢世璧于海龙彭江王玉赵斌张莉汪爱媛眭翔许文静
Owner 卢世璧
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