Method for screening diabetes markers from body fluid metabonome profile
A technology of body fluid metabolism and metabolomics, which is applied in the field of screening small molecular metabolic markers of diabetes, can solve problems such as unreliable modeling results, limited application of double cross-validation methods, poor model interpretation ability, etc., and achieves short sample analysis time. Low operating and maintenance costs and small differences in analysis results
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[0037] Example: Diabetes marker screening based on plasma metabolome profiling.
[0038] (1) Collection and pretreatment of human plasma samples.
[0039] Before the collection, the included volunteers signed the informed consent form, collected under the same sampling conditions, and the collected plasma samples were immediately stored in a -80°C refrigerator. In this embodiment, a total of 119 human plasma samples were collected, including 30 samples from healthy people and 89 samples from diabetic patients.
[0040] Plasma samples were removed from the ultra-low temperature freezer and thawed at room temperature prior to metabolite analysis. Shake for 30 seconds after thawing to mix.
[0041] Take 119 plasma samples respectively, add 300 μL of methanol and internal standard to each 100 μL of plasma, shake for 30 seconds to mix well, and take the supernatant after high-speed centrifugation. After the supernatant was freeze-dried, bis(trimethylsilyl)trifluoroacetamide (BST...
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