Dialysis vessel

Abstract

The invention provides a dialysis vessel. The dialysis vessel comprises a dialysis vessel body and a dialysis vessel cover, wherein the dialysis vessel body is a hollow column; the dialysis vessel cover is closely connected with the upper end of the dialysis vessel body; an air outlet and an air outlet cover are arranged on the dialysis vessel cover; at least two air inlet holes distributed uniformly along the circumferential direction are formed on the inner bottom surface of the dialysis vessel body; the air inlet holes are communicated with an air channel at the bottom of the dialysis vessel body; floating valve plugs are arranged in the air inlet holes; a sealing cover is arranged on an air conduit port at the outer end of the air channel; and at least two supports with wedge-shaped clamping grooves are arranged on the inner bottom surface of the dialysis vessel body along the circumferential direction. By the technical scheme, air bubbling and deoxidizing efficiency can be obviously improved and sealing effect is good, so that oxygen-sensitive samples are protected from oxidative damage in the dialysis process.

Description

technical field [0001] The invention relates to a dialysis apparatus, in particular to a vessel which can be used for anaerobic dialysis operation. Background technique [0002] Dialysis is one of the commonly used techniques in the laboratory, which is used for the replacement of buffer systems, crude purification, concentration, desalination, etc. of macromolecular substances such as proteins. During dialysis, the samples are put into dialysis vessels such as dialysis bags, dialysis tubes, etc., sealed and placed in dialysis fluid for dialysis. [0003] Most proteins can be dialyzed in the dialysate in an open container, but for those samples such as proteins that are easily oxidized, in order to avoid oxidation damage, the sample and dialysate should be kept in anaerobic or oxygen partial pressure during the dialysis process. Crucial in lower conditions. In order to achieve the condition of anaerobic or low oxygen partial pressure, the current measure is to first deoxyg...

AI Technical Summary

Problems solved by technology

However, there is currently no dialysis dish specially used for anaerobic dialysis, and specimen dishes or wide-mouth reagent bottles are generally used instead

When injecting gas into the dialysate in such a vessel, the gas conduit must be extended from the mouth of the vessel to the bottom of the vessel, and the gas can only be discharged from the outlet of the gas conduit, and the lid of the vessel is in a half-open state during the bubbling process. The efficiency is very low, and the oxygen partial pressure is often maintained at a high level after bubbling for a long time; and because the sealing effect of the existing dialysis vessel is poor, the oxygen partial pressure gradually increases during the dialysis process, resulting in oxidative damage to the sample

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