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Rice blast resistance gene Pi7 and application thereof

A technology of resistance gene and rice blast, which is applied in the field of genetic engineering, can solve the problems of lack of effective resistance gene, unresolved, disease-resistant varieties, etc., and achieve the effect of shortening the breeding time

Inactive Publication Date: 2012-11-14
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the diversity and variability of the rice blast fungus population, coupled with the lack of effective use of resistance genes, and the lack of sufficient understanding of the resistance mechanism, the problem of susceptibility of resistant varieties has not been solved, but due to effective The lack of resistance genes and the short life of disease-resistant varieties have become the most difficult problems for breeders

Method used

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  • Rice blast resistance gene Pi7 and application thereof
  • Rice blast resistance gene Pi7 and application thereof
  • Rice blast resistance gene Pi7 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Resistance Gene Pik-h resistance characteristics

[0039] for comparison and clarification Pi7 and Pik Locus 4 alleles ( Pik , Pik-p , Pik-s , Pik-m ) resistance spectrum, using strains from Guangdong (60 strains), Fujian (40 strains), Hunan (41 strains), Guizhou (60 strains), Sichuan (66 strains), Liaoning (108 strains), Jilin (60 strains) and A total of 498 strains collected in Heilongjiang (63 strains) were compared and analyzed for the resistance spectrum of the varieties held by the above five alleles (IRBL7-M, IRBLk-Ka, IRBLkp-K60, IRBLks-S and Tsuyuake, respectively). the result shows, Pi7 Higher resistance to the blast fungus populations in Guangdong, Hunan, Guizhou, Sichuan and Liaoning, indicating that the gene can be used in the above areas ( figure 2 ). Rice varieties RBL7-M, IRBLk-Ka, IRBLkp-K60 and IRBLks-S have been reported in the literature (Kobayashi et al. Development of new sets of international standard differential variet...

Embodiment 2

[0040] Example 2 Rice blast resistance gene Pi7 The cloning strategy and the construction of the electron physical map

[0041] Previous positioning studies have shown Pi7 gene localization with Pik Genomic regions where cluster genes are identical (Campbell et al. Development of co-dominant amplified polymorphic sequence markers in rice that flank the Magnaporthe grisea resistance gene Pi7 (t) in recombinant inbred line 29. Phytopathology, 2004, 94: 302-307; image 3 A); Anti-spectrum analysis in our laboratory shows Pi7 gene and Pik cluster of 3 alleles (except the less resistant Pik-s ) showed the same resistance response to most of the tested strains ( figure 2 ); previous research in our laboratory showed that, with Pi7 corresponding avirulent gene AvrPi7 also with Pik Avirulence gene corresponding to cluster resistance gene AvrPik / km / kp is equivalent. Based on the results of the above three aspects, according to the "gene-to-gene" theory of plant d...

Embodiment 3

[0042] Example 3 Rice blast resistance gene Pi7 Annotation and sequence analysis of candidate genes

[0043] to confirm Pi7 The applicant used the reference sequence of Nipponbare, through three gene prediction software RiceGAAS (http: / / ricegaas.dna.affrc.go.jp), Gramene (http: / / 143.48.220.116 / resources / ) and Softberry FGENESH (http: / / www.softberry.com) performed gene prediction and annotation analysis on the target gene region, and preliminarily determined that Pi7 The candidate resistance genes of 6 nucleotide binding sites and the candidate gene of NBS-LRR (nucleotide binding site - leucine-rich repeat, NBS-LRR) Pi71-NP , Pi72-NP , Pi73-NP , Pi74-NP , Pi75-NP ,and Pi76-NP ).

[0044] Based on the presence / absence (P / A) analysis of 6 candidate genes based on gene-specific markers, the results showed that, compared with Pik cluster of 3 alleles like the 4 candidate genes present in the Nipponbare reference sequence ( Pi71-NP , Pi72-NP , Pi73-NP , Pi7...

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Abstract

The invention discloses a rice blast resistance gene Pi7 and application thereof, and provides a nucleotide sequence of the rice blast resistance gene Pi7 and an amino acid polypeptide sequence coded thereby. The nucleotide sequence comprises two genes which belong to members of a coiled-coil nucleotide binding site leucine-rich repeat (CC-NBS-LRR) resistance gene family and are constitutive expression genes. The invention also provides application of the gene to the aspect of transforming rice or breeding disease-resistant varieties of other plants and application of a molecular marker produced according to the gene sequence to breeding.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to a rice blast resistance gene Pi7 cloning and its application. Background technique [0002] Plants are often attacked by various pathogens during the growth process, and plants adopt various defense strategies to protect themselves and avoid being attacked by them. One of the most important defense mechanisms in plants is the ability to recognize the presence of obligate pathogenic microorganisms and initiate their own defense response system. Plant recognition of pathogenic bacteria is mediated by disease resistance genes. Therefore, the analysis and research on the structure of disease-resistant gene products is the basis for understanding the mechanism of plant disease resistance, and also has important guiding significance for the prevention and control of diseases. [0003] So far, more than 80 disease resistance genes have been isolated from plants. Studies ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/63C12N15/82
Inventor 潘庆华甘霖翟纯林菲王玲
Owner SOUTH CHINA AGRI UNIV
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