Method for culturing high-quality efficient stress-resistant complete set line of large white pigs
A breeding method and technology of supporting lines, which are applied in the field of breeding high-quality and high-efficiency large white pig stress resistance supporting lines, can solve the problem of not establishing an evaluation system for pig immune response ability, etc., so as to improve immune response ability, improve general disease resistance, Increases the effect of special disease resistance
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[0030] 1. Build a basic group
[0031] Using molecular breeding technology and conventional breeding methods, using the genetic markers that have been used so far, to test the halothane gene (RYR1 gene) and the gene related to diarrhea and edema in weaned piglets (FUT1 gene), and eliminate the halothane-carrying pigs. After gene individuals, further according to the FUT1 gene detection results, select FUT1 gene AA allele (resistance type) individuals for matching ( figure 1 ), and the progeny were verified against F18 E. coli infection by in vitro adhesion test and in vivo colonization test (ie, challenge test) ( figure 2 ), and then continue to select and fix the disease-resistant individuals. Focus on the candidate gene strategy, comprehensively consider the traits such as birth weight, weaning weight and growth speed, and establish a basic group of large white pigs that are resistant to diarrhea and edema in weaned piglets.
[0032] The screening of individuals with...
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