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Nanometer vaccine and preparation method thereof

A technology of nano-vaccine and vaccine carrier, which is applied in the field of vaccine and its preparation, can solve the problems of reducing cytotoxicity, etc., and achieve the effect of reducing cytotoxicity, powerful immune adjuvant effect, and improving ability

Inactive Publication Date: 2011-05-25
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of this, it is necessary to address the defects of existing liposome vaccine carriers, and propose a new mannosylated cationic liposome complex, which can be used as a vaccine carrier and applied to vaccines. This new liposome The complex incorporates neutral phospholipids and mannose in cationic lipids, which not only improves the immune adjuvant effect of liposomes and the targeting of antigen-presenting cells, but also significantly reduces its cytotoxicity, providing a highly effective and no side effects The new vaccine carrier and adjuvant can significantly enhance the immune effect of the vaccine

Method used

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  • Nanometer vaccine and preparation method thereof
  • Nanometer vaccine and preparation method thereof
  • Nanometer vaccine and preparation method thereof

Examples

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Embodiment 1

[0048] Example 1: Mannosylated cationic liposome complexes

[0049] The invention proposes to use the mannosylated cationic liposome complex as a novel vaccine carrier to construct a safe and efficient nano-vaccine.

[0050] The structure of the mannosylated cationic liposome complex see figure 1 , comprising cationic lipids, neutral phospholipids and mannoside mixed in proportion, the molar ratio of cationic lipids and neutral phospholipids is 19:1 to 2:18, the molar ratio of mannoside to liposomes comprising cationic lipids and neutral phospholipids The ratio is 2:98 to 20:80.

[0051] Cationic lipids are positively charged amphoteric molecules, including a positively charged head and a hydrophobic tail. The positively charged polar head contains amine groups such as amino groups, quaternary ammonium salts, and polyamines, while the hydrophobic tail includes saturated Or unsaturated fatty acid chain or cholesterol ring, the main chain of the molecule is glycerol, the secon...

Embodiment 2

[0054] Example 2: Vaccine carrier with immune adjuvant effect

[0055] The vaccine carrier with immune adjuvant effect of the present invention wraps vaccine antigen, and the vaccine carrier is mannosylated cationic liposome complex.

[0056] The structure of the mannosylated cationic liposome complex see figure 1 , comprising cationic lipids, neutral phospholipids and mannoside mixed in proportion, the molar ratio of cationic lipids and neutral phospholipids is 19:1 to 2:18, the molar ratio of mannoside to liposomes comprising cationic lipids and neutral phospholipids The ratio is 2:98 to 20:80. Cationic lipids are positively charged amphoteric molecules, including a positively charged head and a hydrophobic tail. The positively charged polar head contains amine groups such as amino groups, quaternary ammonium salts, and polyamines, while the hydrophobic tail includes saturated Or unsaturated fatty acid chain or cholesterol ring, which is an amphipathic molecule with a posi...

Embodiment 3

[0057] Embodiment 3: nano vaccine

[0058] The nano-vaccine of the present invention comprises a vaccine antigen and a liposome encapsulating the vaccine antigen, the liposome adopts mannosylated cationic liposome complex, the antigen is various proteins, polypeptides, polysaccharides, DNA or RNA, and the antigen comes from For viruses, bacteria, other microorganisms, tumors or genetically engineered protein products.

[0059] The structure of the mannosylated cationic liposome complex see figure 1 , comprising cationic lipids, neutral phospholipids and mannoside mixed in proportion, the molar ratio of cationic lipids and neutral phospholipids is 19:1 to 2:18, the molar ratio of mannoside to liposomes comprising cationic lipids and neutral phospholipids The ratio is 2:98 to 20:80. Cationic lipids are positively charged amphoteric molecules, including a positively charged head and a hydrophobic tail. The positively charged polar head contains amine groups such as amino groups...

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Abstract

The invention provides a nanometer vaccine and a preparation method thereof. A new mannose glycosylated-cationic liposome complex can be used as a vaccine vector and can be applied to the vaccine. In the new liposome complex, neutral phospholipid and mannose are mixed into cationic lipid, so the immunologic adjuvant effect and the antigen-presenting cell targeting property of a liposome are improved, and the cytotoxic effect of the liposome is reduced obviously. The invention provides a novel high-efficiency vaccine vector and an adjuvant which do not have side effects and can improve the immunologic effect of the vaccine obviously.

Description

【Technical field】 [0001] The invention relates to a vaccine and a preparation method thereof, in particular to a vaccine using liposome as a carrier and a preparation method thereof. [0002] The present invention also relates to a vaccine carrier. [0003] The invention also relates to a liposome complex applicable to vaccine carriers. 【Background technique】 [0004] Vaccines are an important means to prevent and control the occurrence and development of infectious diseases. Immune adjuvants (also known as non-specific immune proliferators) are indispensable components in vaccines, which can effectively promote specific immune responses induced by vaccines. With the rapid development of vaccine research, new genetically engineered vaccines are being widely used in the research and development of vaccines. This vaccine has the advantages of high purity and strong specificity, but due to its small molecule and relatively poor immunogenicity, it is urgent to combine effecti...

Claims

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Application Information

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IPC IPC(8): A61K47/26A61K9/127A61K39/39A61P31/00
Inventor 马轶凡周冬梅谢晓芳庄燕郑明彬
Owner SHENZHEN INST OF ADVANCED TECH
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